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| WBPicture0000009922 | Description | Figure 2. SCs Visualized by TEM and A-SYP-2 Immunostaining. (A-C) TEM images of nuclei from the late pachytene regions of wild-type and syp-2 germlines.(A) Equatorial section of a wild-type pachytene nucleus containing a very long continuous stretch of SC, visible as a zipper-like track flanked by electron-dense patches of chromatin; the large dark body centered in the upper half of the panel is the nucleolus.(B) Detail of SC structure from a different wild-type nucleus.(C) Equatorial section of a syp-2 nucleus, in which numerous chromatin patches are observed but zipper-like structures are absent. Scale bars equal 500 nm.(D-G) Immunolocalization of SYP-2 throughout meiotic prophase. Images in (D), (E), and (G) show nuclei from whole-mount preparations of wild-type germlines; images in (F) show two halves of a single nucleus prepared using a squash procedure. DAPI-stained chromosomes are in blue; A-SYP-2 is in red.(D) Field of nuclei that spans entry into meiotic prophase; progression is from left to right. The first nuclei in which SYP-2 is detected contain a single bright SYP-2 focus; these are followed by nuclei with several chromosomal foci of SYP-2, then by nuclei with extended chromosome-associated patches of SYP-2 by mid-transition zone.(E) Pachytene nuclei, exhibiting continuous SYP-2 localization at the interface between aligned homologous chromosomes; images are projections halfway through data stacks encompassing whole nuclei.(F) Nucleus exhibiting extensive synapsis over most chromosome segments (indicated by tight association of parallel DAPI tracks flanking A-SYP-2 staining) plus a region in which corresponding segments of homologs are not closely juxtaposed and lack A-SYP-2 staining (arrow).(G) Three nuclei at the diakinesis stage, in which chromosomes are highly compact and individual bivalents are clearly resolved. SYP-2 progressively dissociates from chromosomes during diakinesis and is no longer detected by late diakinesis (progression is from left to right). Scale bars equal 2 um. | |||
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| Name | S2.jpg | ||||
| Crop | Crop_picture | WBPicture0000009923 | |||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1016/S1534-5807(03)00232-6 | ||
| Journal_URL | DevelopmentalCell | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00006086 |
