WormBase Tree Display for Picture: WBPicture0000008940

WBPicture0000008940 Description: Figure 4. HIM-10 localizes to the kinetochore region of the kinetochore-centromere complex. All panels are false-color confocal images. Interpretative cartoons (right) represent images from D, G, K, and O. (A, H, and L) Wild-type embryos co-stained with DAPI (red) and anti-HIM-10 antibodies (green). (A) HIM-10 associates with prophase (arrows) but not interphase (arrowhead) chromosomes. (B-D) Enlargement of nucleus in A. In cross section, HIM-10 appears as paired dots flanking opposite sides of a prophase chromosome. (E-G) Enlargement of nucleus in A. HIM-10 forms two tracks flanking a prophase chromosome. (H) HIM-10 appears depleted from the cytoplasm of cells in metaphase. (I-K) Enlargement of metaphase cell (arrow) in H. HIM-10 appears as stripes associated with each poleward face of the metaphase plate. (L) An embryo with HIM-10 localized to the poleward face of anaphase chromosomes. (M-O) Enlargement of anaphase figure (arrow) in L. HIM-10 appears as many individual dots along the chromatin. These dots may represent the kinetochores associated with chromosomes moving at slightly different rates to the spindle pole. (P-X) Wild-type animals costained with DAPI (blue) and antibodies to both the centromeric marker HCP-3 (red) and HIM-10 (green). (P-R) Prophase nucleus from the mitotic region of the hermaphrodite gonad. (P and Q) Both proteins localize in two parallel tracks flanking the replicated chromosomes. (R) The HIM-10 signal overlaps with (yellow) and extends more distal to (green) the HCP-3 signal. In metaphase (S-U) and anaphase (V-X) of embryos, both proteins localize to the poleward face of the chromatin. In the merged images (U and X) HIM-10 overlaps with (yellow) and extends closer to the pole than (green) HCP-3. Bars, 2 um.
  Name: F4.large.jpg
  Depict: Expr_pattern: Expr1357
    Cellular_component: GO:0000776
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. The user must not use the Work for commercial purposes. If the user shares, alters, transforms, or builds upon the Work, the user may distribute the resulting work only under the same license the user received from <Publisher_URL>.
    Publication_year: 2001
    Article_URL: DOI id 10.1083/jcb.153.6.1227
    Journal_URL: TheJournalofCellBiology
    Publisher_URL: TheRockefellerUniversityPress
  Reference: WBPaper00004737