WormBase Tree Display for Picture: WBPicture0000008940
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| WBPicture0000008940 | Description | Figure 4. HIM-10 localizes to the kinetochore region of the kinetochore-centromere complex. All panels are false-color confocal images. Interpretative cartoons (right) represent images from D, G, K, and O. (A, H, and L) Wild-type embryos co-stained with DAPI (red) and anti-HIM-10 antibodies (green). (A) HIM-10 associates with prophase (arrows) but not interphase (arrowhead) chromosomes. (B-D) Enlargement of nucleus in A. In cross section, HIM-10 appears as paired dots flanking opposite sides of a prophase chromosome. (E-G) Enlargement of nucleus in A. HIM-10 forms two tracks flanking a prophase chromosome. (H) HIM-10 appears depleted from the cytoplasm of cells in metaphase. (I-K) Enlargement of metaphase cell (arrow) in H. HIM-10 appears as stripes associated with each poleward face of the metaphase plate. (L) An embryo with HIM-10 localized to the poleward face of anaphase chromosomes. (M-O) Enlargement of anaphase figure (arrow) in L. HIM-10 appears as many individual dots along the chromatin. These dots may represent the kinetochores associated with chromosomes moving at slightly different rates to the spindle pole. (P-X) Wild-type animals costained with DAPI (blue) and antibodies to both the centromeric marker HCP-3 (red) and HIM-10 (green). (P-R) Prophase nucleus from the mitotic region of the hermaphrodite gonad. (P and Q) Both proteins localize in two parallel tracks flanking the replicated chromosomes. (R) The HIM-10 signal overlaps with (yellow) and extends more distal to (green) the HCP-3 signal. In metaphase (S-U) and anaphase (V-X) of embryos, both proteins localize to the poleward face of the chromatin. In the merged images (U and X) HIM-10 overlaps with (yellow) and extends closer to the pole than (green) HCP-3. Bars, 2 um. |
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| Name | F4.large.jpg | |
| Depict (2) | ||
| Acknowledgment (5) | ||
| Reference | WBPaper00004737 |
