WormBase Tree Display for Expr_pattern: Expr1357
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| Expr1357 | Expression_of | Gene | WBGene00001869 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001869 | ||
| Expression_data | GO_term | GO:0000776 | |
| Subcellular_localization | Confocal microscopy of animals stained with HIM-10 antibodies showed HIM-10 to be localized in a pattern expected for kinetochore proteins on holocentric chromosomes. HIM-10 antibody staining was not detected on chromosomes of interphase nuclei. During prophase, HIM-10 was visualized as parallel tracks (in longitudinal sections) or paired dots (in cross sections) flanking each mitotic prophase chromosome. At metaphase, HIM-10 was distributed in stripes along the poleward faces of the chromatin at the metaphase plate. During anaphase, one poleward-facing stripe was associated with each set of sister chromatids. Antibody staining was not detected during telophase. These staining patterns were most evident in the large cells of the early embryo but were also seen in the mitotic germline. HIM-10 antibody staining was not consistently seen on other structures in embryos or the mitotic germline, but a recurrent suggestion of spindle and centrosome staining was found with some antibody preparations. | ||
| Type | Antibody | Rabbit or rat polyclonal antisera against recombinant protein of HIM-10. | |
| Picture | WBPicture0000008940 | ||
| Remark | No detail described on cellular expression patterns. | ||
| Reference | WBPaper00004737 | ||
| Antibody_info | WBAntibody00000382 | ||
| WBAntibody00000383 |
