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WBPicture0000008387DescriptionFigure 5. HIM-3 associates with the chromosome core. (A-C) Immunofluorescence micrographs of squashed meiotic prophase chromosomes assembling lateral elements and SC stained with anti-HIM-3. (A) Arrow shows the formation of a stretch of lateral element early in prophase that becomes contiguous in B as the chromosomes continue to condense. The diffuse appearance of the DAPI staining is probably the result of a variation in the fixation time. (C) HIM-3 colocalizes with the synaptonemal complex of the pachytene chromosomes. Scale bars, 10 μm. (D-E) Chromosomes at diakinesis stained with anti-HIM-3 showing that HIM-3 remains at the chromosome core after desynapsis. Scale bar, 10 μm. (E) A magnification of a single bivalent where HIM-3 is associated with the chromosome core and is not present at the junction of the bivalent occupied by the chiasma (arrowhead). Scale bar, 1 μm.
NameF5.large.jpg
DepictExpr_patternExpr1163
Cellular_componentGO:0000794
AcknowledgmentTemplateWormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1999
Article_URLDOIid10.1101/gad.13.17.2258
Journal_URLGenes&Development
Publisher_URLColdSpringHarborLabPress
ReferenceWBPaper00003675