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WBPicture0000008387DescriptionFigure 5. HIM-3 associates with the chromosome core. (A-C) Immunofluorescence micrographs of squashed meiotic prophase chromosomes assembling lateral elements and SC stained with anti-HIM-3. (A) Arrow shows the formation of a stretch of lateral element early in prophase that becomes contiguous in B as the chromosomes continue to condense. The diffuse appearance of the DAPI staining is probably the result of a variation in the fixation time. (C) HIM-3 colocalizes with the synaptonemal complex of the pachytene chromosomes. Scale bars, 10 μm. (D-E) Chromosomes at diakinesis stained with anti-HIM-3 showing that HIM-3 remains at the chromosome core after desynapsis. Scale bar, 10 μm. (E) A magnification of a single bivalent where HIM-3 is associated with the chromosome core and is not present at the junction of the bivalent occupied by the chiasma (arrowhead). Scale bar, 1 μm.
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ReferenceWBPaper00003675