WormBase Tree Display for Expr_pattern: Expr1163
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| Expr1163 | Expression_of | Gene | WBGene00001862 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001862 | |||
| Expression_data | Life_stage | WBls:0000035 | ||
| Anatomy_term | WBbt:0006797 | Certain | ||
| WBbt:0006799 | Certain | |||
| GO_term | GO:0000793 | |||
| Subcellular_localization | Nuclei. The him-3 protein localizes to the chromosome core and associates with both unsynapsed and synapsed chromosomes. | |||
| Type | Antibody | L3 larvae and microdissected gonads from wild-type hermaphrodites and males were stained with an antibody raised against the carboxy-terminal portion of the HIM-3 protein. | ||
| Pattern | All three gamete types of C. elegans (oocyte, male spermatocyte, and hermaphrodite spermatocyte) show meiosis-specific expression of HIM-3. No protein could be detected in somatic nuclei at any stage of development or in the distal tip nuclei that are undergoing mitotic divisions. A strong signal first appears in the transition zone where HIM-3 is associated with the chromatin of early meiotic prophase nuclei. Localization of HIM-3 becomes more discrete in pachytene and diplotene as the chromosomes synapse and desynapse. The protein remains associated with the chromosomes of oocytes at diakinesis; however, it could no longer be detected in postmeiotic sperm. The antibody identifies the first nuclei that are entering the pachytene stage of meiosis but does not stain the mitotic nuclei. | |||
| Picture | WBPicture0000008385 | |||
| WBPicture0000008386 | ||||
| WBPicture0000008387 | ||||
| Reference | WBPaper00003675 | |||
| Antibody_info | WBAntibody00000238 |
