WormBase Tree Display for Interaction: WBInteraction000542004

WBInteraction000542004 Interaction_type: Regulatory: Change_of_expression_level
  Interactor: Interactor_overlapping_gene: WBGene00006794 Interactor_type: Trans_regulated
        Expr_pattern: Expr1864
        Antibody: WBAntibody00000222
      WBGene00006321 Interactor_type: Trans_regulator
    Variation_interactor: WBVar00248713 Interactor_type: Trans_regulator
      WBVar00248736 Interactor_type: Trans_regulator
  Interaction_summary: The protein level of UNC-60B, the muscle-specific isoform, was greatly reduced in the sup-12 mutants. In contrast, the total level of UNC-60A, the nonmuscle isoform, was not significantly altered by mutations in sup-12 or unc-60B. Similar changes were detected at the mRNA levels: the unc-60B mRNA was reduced as compared with wild type, whereas the unc-60A mRNA was not significantly altered. In wild-type background, the unc-60B mRNA was decreased in the sup-12 mutants. In wild type and the unc-60B(e677) single mutant, UNC-60A was not detectable in body wall muscle when worms were stained for UNC-60A. However, in the unc-60B;sup-12 double mutant and the sup-12 single mutant, UNC-60A protein was detected in the diffuse cytoplasm.
  Detection_method: Antibody
    In_situ
    Northern
    Western: [cgc3529]:unc-60_a and [cgc3529]:unc-60_b
  Regulation_level: Post_transcriptional
  Regulation_result: Negative_regulate: Subcellular_localization: cytoplasma
  Paper: WBPaper00024604
  Remark: UNC-60A is expressed in a variety of nonmuscle cells. Therefore, changes in the level of UNC-60A in a subset of tissues may not be detected by the Northern blot analysis. The sup-12 mutations have opposite effects on the levels of the two splice variants, unc-60A and unc-60B, in muscle cells. The suppression of the Unc-60 phenotype by sup-12 could be explained by the up-regulation of UNC-60A in muscle, which may compensate for the function of UNC-60B.