WormBase Tree Display for Expr_pattern: Expr1864
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| Expr1864 | Expression_of | Gene | WBGene00006794 | |
|---|---|---|---|---|
| WBGene00002978 | ||||
| Reflects_endogenous_expression_of | WBGene00002978 | |||
| WBGene00006794 | ||||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0003675 | Certain | ||
| GO_term | GO:0005737 | |||
| GO:0005865 | ||||
| Subcellular_localization | Both UNC-60B and CeTM became detectable in muscle cells at a very early stage of muscle development (~290 min), nearly at the same time. At the comma stage (290350 min), both UNC-60B and CeTM are diffusely localized in the cytoplasm and positions of nuclei are devoid of staining. After the 1.5-fold stage (~430 min), CeTM was gradually localized to a narrow region which represented myo-fibrils as determined by double staining with anti-actin antibody, whereas UNC-60B remained in the diffuse cytoplasm. This differential localization of UNC-60B and CeTM was maintained throughout the later embryonic development. In adult body wall muscle, CeTM and UNC-60B were also localized to different regions of thin filaments. Interestingly, the staining patterns of CeTM and actin were different. CeTM was localized in linear patterns, whereas actin was localized in ladder-like patterns in which the regions of dense bodies were devoid of staining. UNC-60B was stained as dotted lines between two lines of CeTM with little overlapping zones. Double staining of actin and UNC-60B showed that UNC-60B was localized to the central areas of the actin ladders between dense bodies. Double staining of vinculin, as a marker for dense bodies, and UNC-60B revealed that UNC-60B filled the gaps between dense bodies but was not colocalized with them. The shape of the UNC-60Bpositive dots was irregular, suggesting that association of UNC-60B with myofibrils is unstable and dynamic. | |||
| Type | Antibody | Anti-UNC-60 antibody and anti-CeTM antibody. | ||
| Pattern | Expressed in muscle. | |||
| Picture | WBPicture0000008950 | |||
| WBPicture0000008951 | ||||
| Reference | WBPaper00005168 | |||
| Antibody_info | WBAntibody00000223 | |||
| WBAntibody00000472 |
