WormBase Tree Display for Interaction: WBInteraction000501479
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| WBInteraction000501479 | Interaction_type | Regulatory | |||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00006742 | Interactor_type | Trans_regulator | |
| WBGene00006600 | Interactor_type | Trans_regulated | |||
| Expr_pattern | Expr959 | ||||
| Variation_interactor | WBVar00142938 | Interactor_type | Trans_regulator | ||
| Interaction_summary | Temperature shift experiments were performed using unc-2;daf-4(m592) double mutants to identify the developmental period during which thermal enhancement of tph-1::GFP expression requires UNC-2 regulation of TGF-b signaling. Animals grown to the L3 larval stage or beyond at 15 Centigrades did not develop enhanced expression of the tph-1::GFP transgene when shifted to 25 Centigrades and, conversely, animals grown to the L3 larval stage at 25 C retained high expression of the tph-1::GFP transgene in the ADF neurons when downshifted to 15 Centigrades. This experiment demonstrates a developmental window during the L2 larval stage, around the time at which the decision is made to commit to dauer larva development, beyond which thermal inactivation of DAF-4 will not change the percentage of ADF neurons with increased expression of the tph-1::GFP transgene. Together these data show that UNC-2 and the TGF-b pathway transduce a developmental thermal cue regulating expression of tph-1. | ||||
| Detection_method | Reporter_gene | [tph-1::gfp] | |||
| Regulation_result | Positive_regulate | Life_stage | WBls:0000027 | ||
| Anatomy_term | WBbt:0003999 | ||||
| WBbt:0004003 | |||||
| Paper | WBPaper00006272 |
