WormBase Tree Display for Interaction: WBInteraction000501477
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| WBInteraction000501477 | Interaction_type | Regulatory | |||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00006742 | Interactor_type | Trans_regulator | |
| WBGene00006600 | Interactor_type | Trans_regulated | |||
| Expr_pattern | Expr959 | ||||
| Variation_interactor | WBVar00142938 | Interactor_type | Trans_regulator | ||
| Interaction_summary | In C. elegans the serotonergic neurons all express tph-1. Comparison of the expression of a tph-1 promoter::GFP transgene in fed WT, unc-2, unc-2; daf-4 and unc-2;unc-43(gf) adult animals grown at 25 centigrades showed WT levels of expression in unc-2;daf-4 and unc-2;unc-43(gf), and relatively decreased expression in the ADF neurons of unc-2, suggesting that unc-2 differences in 5-HT staining were due to transcriptional regulation of TPH-1 levels but not failure to differentiate. | ||||
| Detection_method | Reporter_gene | [tph-1::gfp] | |||
| Regulation_result | Positive_regulate | Anatomy_term | WBbt:0003999 | ||
| WBbt:0004003 | |||||
| Paper | WBPaper00006272 | ||||
| Remark | To confirm that these changes in tph-1::GFP expression level were not due to a failure of monoaminergic differentiation, the expression of the vesicular monoamine transporter gene cat-1 was compared in the ADF neurons of WT and unc-2 animals using a cat-1 promoter::GFP transgene. Comparison of these strains revealed that their cat-1::GFP expression patterns were indistinguishable, suggesting that the differences in tph-1::GFP expression in unc-2 are caused by the absence of an UNC-2-dependent signal required after serotonergic differentiation. |
