| WBGene00010160 | SMap | S_parent | Sequence | CHROMOSOME_I |
|---|
| Identity | Version | 2 |
| | Name | CGC_name | png-1 | Person_evidence | WBPerson114 |
| | | Sequence_name | F56G4.5 |
| | | Molecular_name | F56G4.5 |
| | | | F56G4.5.1 |
| | | | CE23786 |
| | | Other_name | bam-1 |
| | | | CELE_F56G4.5 | Accession_evidence | NDB | BX284601 |
| | | Public_name | png-1 |
| | DB_info | Database (13) |
| | Species | Caenorhabditis elegans |
| | History | Version_change | 1 | 26 May 2004 16:54:51 | WBPerson1971 | Event | Imported | Initial conversion from CDS class of WS125 |
| | | | 2 | 15 Mar 2007 17:00:31 | WBPerson2970 | Name_change | CGC_name | png-1 |
| | Status | Live |
| Gene_info | Biotype | SO:0001217 |
| | Gene_class | png |
| | Allele (152) |
| | Strain | WBStrain00001991 |
| | | WBStrain00030529 |
| | | WBStrain00032150 |
| | RNASeq_FPKM (74) |
| | GO_annotation (25) |
| | Contained_in_operon | CEOP1652 |
| | Ortholog (35) |
| | Paralog (15) |
| | Structured_description | Concise_description | png-1 encodes a peptide-N[4]-(N-acetyl-beta-D-glucosaminyl) asparagineamidase (i.e., peptide:N-glycanase or PNGase) orthologous to buddingyeast PNG1 and human NGLY1; PNG-1 is atypically organized, with anN-terminal thioredoxin-like domain, a central transglutaminase domain,and a C-terminal DUF750 domain, while lacking the PUB domain found inmammalian or insect PNGases; both in vitro and in vivo, PNG-1 shows bothprotein disulfide reductase and PNGase activities, which require itsN-terminal and central domains respectively; PNG-1 has PNGase activityon denatured but not natively folded RNAse B, and releases both highmannose- and sialo-complex-type N-glycans from glycopeptides anddenatured glycoproteins; in vivo, the png-1(cy8) mutation C251Y(predicted to destroy the PNGase active site) abolishes PNGase activitywhile leaving PNG-1's reductase activity intact; conversely, PNG-1'sreductase activity is abolished by the dual missense mutation C34S/C37S,which alters its predicted N-terminal active site; by orthology, PNG-1is expected to aid proteasomal degradation of misfolded proteins; PNG-1activity is inhibited by Zn(2+) and the caspase inhibitor z-VAD-fmk, butnot by EDTA; PNG-1 is expressed in renal gland cells and intestine;although W04G5.5 and Y50D4B.7 have some similarity to thetransglutaminase domain of PNG-1, they lack a predicted catalytic triadactive site. | Paper_evidence | WBPaper00006525 |
| | | | | | WBPaper00029398 |
| | | | | | WBPaper00029436 |
| | | | | Curator_confirmed | WBPerson567 |
| | | | | Date_last_updated | 05 Jun 2007 00:00:00 |
| | | Automated_description | Enables peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity and protein-disulfide reductase (NAD(P)) activity. Involved in cell redox homeostasis; negative regulation of collateral sprouting; and ubiquitin-dependent ERAD pathway. Located in cytoplasm. Expressed in body wall musculature; gonad; neurons; pharynx; and vulva. Human ortholog(s) of this gene implicated in NGLY1-deficiency. Is an ortholog of human NGLY1 (N-glycanase 1). | Paper_evidence | WBPaper00065943 |
| | | | | Curator_confirmed | WBPerson324 |
| | | | | | WBPerson37462 |
| | | | | Inferred_automatically | This description was generated automatically by a script based on data from the WS291 version of WormBase |
| | | | | Date_last_updated | 29 Nov 2023 00:00:00 |
| Disease_info | Potential_model | DOID:0060728 | Homo sapiens | Inferred_automatically | Inferred by orthology to human genes with DO annotation (HGNC:17646) |
| Molecular_info | Corresponding_CDS | F56G4.5 |
| | Corresponding_transcript | F56G4.5.1 |
| | Other_sequence | EX539623.1 |
| | | Acan_isotig11424 |
| | | CBC00976_1 |
| | | MAC00959_1 |
| | | ACC06749_1 |
| | | PT03448 |
| | | SR04827 |
| | | Dviv_isotig29313 |
| | | MA01872 |
| | Associated_feature | WBsf656941 |
| | | WBsf985278 |
| | | WBsf1010709 |
| | | WBsf218531 |
| Experimental_info | RNAi_result | WBRNAi00116961 | Inferred_automatically | RNAi_primary |
| | | WBRNAi00076493 | Inferred_automatically | RNAi_primary |
| | | WBRNAi00048764 | Inferred_automatically | RNAi_primary |
| | | WBRNAi00032982 | Inferred_automatically | RNAi_primary |
| | | WBRNAi00004720 | Inferred_automatically | RNAi_primary |
| | Expr_pattern | Expr6232 |
| | | Expr8899 |
| | | Expr8900 |
| | | Expr1024009 |
| | | Expr1034438 |
| | | Expr1152511 |
| | | Expr2014983 |
| | | Expr2033218 |
| | Drives_construct | WBCnstr00002775 |
| | | WBCnstr00013592 |
| | | WBCnstr00013594 |
| | Construct_product | WBCnstr00013594 |
| | Microarray_results (21) |
| | Expression_cluster | WBPaper00033065:cyc-1(RNAi)_upregulated |
| | | WBPaper00044501:gld-1_let-7_regulated |
| | | WBPaper00044736:flat_dev_expression |
| | | WBPaper00044760:somatic_specific |
| | | WBPaper00045257:bar-1(ga80)_downregulated |
| | | WBPaper00045420:fertilization_downregulated_transcript |
| | | WBPaper00045521:Gender_Neutral |
| | | WBPaper00045774:clk-1_downregulated |
| | | WBPaper00048923:Aging_regulated |
| | | WBPaper00048926:miaserin_upregulated_adult-day5 |
| | | WBPaper00048988:daf-16(mu86)_upregulated_neuron |
| | | WBPaper00048988:neuron_expressed |
| | | WBPaper00049545:cco-1(RNAi)_downregulated |
| | | WBPaper00049545:jmjd-3.1(+)_downregulated |
| | | WBPaper00050163:chd-3(eh4);let-418(RNAi)_downregulated_24-cell-embryo |
| | | WBPaper00050370:dpy-21(e428)_L3_downregulated |
| | | WBPaper00050488:adult_vs_dauer_regulated_N2_20C |
| | | WBPaper00050488:N2_vs_mir-34(gk437)_regulated_dauer_20C |
| | | WBPaper00050726:OsmoticStress_regulated_Food |
| | | WBPaper00050726:OsmoticStress_regulated_NoFood |
| | | WBPaper00050990:intestine_expressed |
| | | WBPaper00050990:NMDA-neuron_expressed |
| | | WBPaper00051039:germline_enriched |
| | | WBPaper00051245:epidermis_cytoplasm_expressed |
| | | WBPaper00051245:intestine_cytoplasm_expressed |
| | | WBPaper00051245:pharyngeal-muscle_cytoplasm_expressed |
| | | WBPaper00052884:emb-4(hc60)_downregulated |
| | | WBPaper00053184:sma-2(rax5)_downregulated |
| | | WBPaper00053184:sma-4(rax3)_downregulated |
| | | WBPaper00053302:zidovudine_24h_regulated |
| | | WBPaper00053388:dauer_regulated_Cluster5 |
| | | WBPaper00055354:Rapamycin-Rifampicin-Psora_upregulated |
| | | WBPaper00055354:Rapamycin-Rifampicin_upregulated |
| | | WBPaper00055971:nhl-2(ok818)_25C_upregulated |
| | | WBPaper00058598:sin-3(tm1276)_downregulated |
| | | WBPaper00059328:mrps-5(RNAi)_upregulated_mRNA |
| | | WBPaper00060014:set-2(zr2012)_upregulated |
| | | WBPaper00060661:sensory-neuron_enriched |
| | | WBPaper00061203:sin-3(tm1276)_upregulated |
| | | WBPaper00061340:M1_parent |
| | | WBPaper00061479:HDA-1_interacting_protein |
| | | WBPaper00061527:R119.3_3379-fars-2_2630 |
| | | WBPaper00062159:hda-2(ok1479)_upregulated |
| | | WBPaper00062325:muscle_enriched_coding-RNA |
| | | WBPaper00064071:NHR-49_interacting |
| | | WBPaper00064130:Beta-Amyloid_downregulated_protein |
| | | WBPaper00064494:postdauer_downregulated_AWA |
| | | WBPaper00064716:paraquat_upregulated |
| | | WBPaper00065373:Cisplatin_downregulated_WT |
| | | WBPaper00065373:sek-1(km4)_downregulated_Ref |
| | | cgc4386_cluster_4_5 |
| | | cgc4489_group_11 |
| | | WBPaper00025032:cluster_195 |
| | | WBPaper00025032:PAL-1_target_genes |
| | | WBPaper00025141:N2_Expressed_Genes |
| | | WBPaper00025141:unc-4::GFP_Expressed_Genes |
| | | WBPaper00026980:intestine_enriched |
| | | WBPaper00035905:FBF-1_Associated |
| | | WBPaper00036286:Pattern_H |
| | | WBPaper00036375:depleted_in_PVD_OLL |
| | | WBPaper00041002:HF_11d_2.0mM_Up |
| | | WBPaper00041606:CE_S.marcescens_regulated |
| | | WBPaper00041606:CE_X.nematophila_regulated |
| | | WBPaper00041939:control_vs_UVC-EtBr-exposed_3h |
| | | WBPaper00041939:control_vs_UVC-EtBr-exposed_51h |
| | | WBPaper00041939:control_vs_UVC-exposed_3h |
| | | WBPaper00041939:control_vs_UVC-exposed_51h |
| | | WBPaper00041939:EtBr-exposed_vs_UVC-exposed_3h |
| | | WBPaper00041939:EtBr-exposed_vs_UVC-exposed_51h |
| | | WBPaper00041939:UVC-EtBr-exposed_vs_EtBr-exposed_3h |
| | | WBPaper00041939:UVC-EtBr-exposed_vs_EtBr-exposed_48h |
| | | WBPaper00041939:UVC-EtBr-exposed_vs_EtBr-exposed_51h |
| | | WBPaper00045960:L1-L4-lethargus_downregulated |
| | | WBPaper00045960:L4-lethargus_downregulated |
| | | WBPaper00048989:eat-2(ad465)_rapamycin_upregulated |
| | | WBPaper00048989:N2_allantoin_upregulated |
| | | WBPaper00053236:Starvation_regulated_GR1307 |
| | | WBPaper00053236:Starvation_regulated_N2 |
| | | [cgc5767]:expression_class_E |
| | | [cgc5767]:expression_class_E_pi(186_min) |
| | | [cgc5767]:expression_class_M |
| | | [cgc5767]:expression_class_MD |
| | | [cgc5767]:expression_class_MDE |
| | | [cgc5767]:expression_class_MD_pd(PC32) |
| | | [cgc5767]:expression_class_ME |
| | Interaction (21) |
| Map_info | Map | I | Position | 7.95669 | Error | 0.002444 |
| | Positive | Positive_clone | F56G4 | Inferred_automatically | From sequence, transcript, pseudogene data |
| | Pseudo_map_position |
| Reference (17) |
| Remark | Map position created from combination of previous interpolated map position (based on known location of sequence) and allele information. Therefore this is not a genetic map position based on recombination frequencies or genetic experiments. This was done on advice of the CGC. | CGC_data_submission |
| Method | Gene |
