[Morgan PG] fc21 exhibits increased sensitivity to a variety of volatile anesthetics. Also ts lethal, with brood size of 10 - 20 at 25C, 50 - 100 at 20C. fc21/Df almost lethal, brood size 5 - 10. Probable hypomorph. Encodes NADH-ubiquinone reductase. Predicted gene K09A9.5
The gas-1 gene encodes a 49 kDa subunit of mitochondrial complex I that is required for oxidative phosphorylation, resistance to volatile anesthetics, fecundity, and normal lifespan; gas-1 is expressed in the nematode neuromuscular system; its subcellular distribution appears to be mitochondrial; drug effects on mutants versus wild-type indicate that gas-1 functions at least partially through presynaptic effects; gas-1 mutants have strongly reduced Complex I-dependent metabolism in mitochondria, while Complex II-dependent metabolism is conversely increased in mutants; gas-1 mitochondria look structurally normal.
Predicted to enable several functions, including 4 iron, 4 sulfur cluster binding activity; NAD binding activity; and NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone and response to xenobiotic stimulus. Located in intracellular membrane-bounded organelle. Expressed in anterior gonad arm; body wall musculature; nervous system; non-striated muscle; and tail. Used to study alcohol use disorder and mitochondrial complex I deficiency. Human ortholog(s) of this gene implicated in several diseases, including Leigh disease; multiple sclerosis; and nuclear type mitochondrial complex I deficiency 6. Is an ortholog of human NDUFS2 (NADH:ubiquinone oxidoreductase core subunit S2).