WormBase Tree Display for Construct: WBCnstr00007204
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WBCnstr00007204 | Summary | [Pgtl-1(7)::gfp] | |
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Driven_by_gene | WBGene00001795 | ||
Fusion_reporter | GFP | ||
Construction_summary | The gtl-1 promoter fragment that contained 2876 bp of the 5' upstream region and 21 bp of the first exon was amplified by PCR and cloned into pPD95.77 (a GFP expression vector, a gift from A. Fire) using the Gateway cloning system (Invitrogen); the GFP coding frame was fused with the 7th amino acid of the GTL-1 full-length protein, and the promoter region contained the 1651 bp upstream from the initiation condon (the upstream gene C05C12.5 starts at 1651 bp from this codon) (Teramoto et al., 2005). | ||
Used_for | Transgene_construct | WBTransgene00007390 | |
Reference | WBPaper00026597 |