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WormBase Tree Display for Construct: WBCnstr00007204

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Name Class

WBCnstr00007204Summary[Pgtl-1(7)::gfp]
Driven_by_geneWBGene00001795
Fusion_reporterGFP
Construction_summaryThe gtl-1 promoter fragment that contained 2876 bp of the 5' upstream region and 21 bp of the first exon was amplified by PCR and cloned into pPD95.77 (a GFP expression vector, a gift from A. Fire) using the Gateway cloning system (Invitrogen); the GFP coding frame was fused with the 7th amino acid of the GTL-1 full-length protein, and the promoter region contained the 1651 bp upstream from the initiation condon (the upstream gene C05C12.5 starts at 1651 bp from this codon) (Teramoto et al., 2005).
Used_forTransgene_constructWBTransgene00007390
ReferenceWBPaper00026597