WormBase Tree Display for Transgene: WBTransgene00007390
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WBTransgene00007390 | Public_name | WBPaper00026597Ex1 | |
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Summary | [Pgtl-1(7)::gfp] | ||
Construction | Construct | WBCnstr00007204 | |
Construction_summary | The gtl-1 promoter fragment that contained 2876 bp of the 5 upstream region and 21 bp of the first exon was amplified by PCR and cloned into pPD95.77 (a GFP expression vector, a gift from A. Fire) using the Gateway cloning system (Invitrogen); the GFP coding frame was fused with the 7th amino acid of the GTL-1 full-length protein, and the promoter region contained the 1651 bp upstream from the initiation condon (the upstream gene C05C12.5 starts at 1651 bp from this codon) (Teramoto et al., 2005). | ||
Genetic_information | Extrachromosomal | ||
Reference | WBPaper00026597 | ||
Species | Caenorhabditis elegans |