WormBase Tree Display for Transcript: F56H9.4a
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| F56H9.4a | SMap | S_parent | Sequence | F56H9 | ||
|---|---|---|---|---|---|---|
| Structure | Source_exons | 1 | 151 | |||
| 205 | 247 | |||||
| 366 | 507 | |||||
| 766 | 938 | |||||
| 987 | 1115 | |||||
| 1169 | 1981 | |||||
| 2028 | 2181 | |||||
| 2247 | 2368 | |||||
| 2412 | 2474 | |||||
| DB_info | DB_remark | non-protein coding RNA gpa-9 | ||||
| Database | RNAcentral | URSid | URS00008553BF | |||
| Origin | From_laboratory | HX | ||||
| Species | Caenorhabditis elegans | |||||
| Visible | Gene | WBGene00001671 | ||||
| Corresponding_PCR_product | cenix:163-f2 | |||||
| mv_F56H9.4 | ||||||
| sjj_F56H9.4 | ||||||
| Corresponding_oligo_set (13) | ||||||
| RNAi_result | WBRNAi00048793 | |||||
| WBRNAi00015855 | ||||||
| Microarray_results (14) | ||||||
| Brief_identification | non-coding Transcript Isoform | |||||
| Last_reviewed | 13 Jul 2015 00:00:00 | WBPerson4025 | ||||
| Remark | [981116 jd] : gene structure altered after email from Gert Janson (gjans@nik.nl) | |||||
| [110720 gw3] There is evidence from SwissProt and human proteins for this isoform. Added a new intron in the fourth exon based on EST data. | Accession_evidence | NDB | FR830797 | |||
| Curator_confirmed | WBPerson4025 | |||||
| [141009 gw3] Converted this from a CDS isoform to a non-coding transcript isoform because there is no evidence that the long sixth exon is spliced correctly to make a CDS. | Curator_confirmed | WBPerson4025 | ||||
| From_analysis | RNASeq | |||||
| [150713 gw3] Discussed the structure of this gene with Gert Jansen. He says: "gpa-9 is indeed pretty odd. Cuppen et al (2003, Comp Funct Genom 2003; 4: 479-491) could not clone the 5' end of the cDNA. We have no real data on gpa-9 structure. There is some functional data on gpa-9, but not much and I agree that this does not really prove it functions as a G alpha subunit. The best we have is that a GFP fusion that fuses gpa-9 in frame at +44 to GFP shows GFP in a number of cells. This construct was generated using primers: taagatctACTTAACCTCCACCTGTTTCT atactgcagCTCGGGAACTTCTGTGATTCA And there is a paper (referred to in Wormbase) that shows that the knock out (without rescue) and overexpression of gpa-9 (genomic constructs driven by daf-28 promoter or endogenous gpa-9 promoter) affect ageing. | Person_evidence | WBPerson291 | ||||
| Curator_confirmed | WBPerson4025 | |||||
| Properties | Transcript | ncRNA | ||||
| Isoform | Curator_confirmed | WBPerson4025 | ||||
| Method | non_coding_transcript |
