WormBase Tree Display for Expr_pattern: Expr982
expand all nodes | collapse all nodes | view schema
| Expr982 | Expression_of | Gene | WBGene00002227 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002227 | ||
| Expression_data | Life_stage | WBls:0000003 | |
| GO_term | GO:0005634 | ||
| Subcellular_localization | nucleus | ||
| Type | In_situ | Using a KLP-17-specific cDNA probe. | |
| Pattern | klp-17 RNA is expressed quite specifically in the nucleus of the cells during C. elegans embryogenesis. The nuclear expression of klp-17 appears as early as the one-cell stage embryo, and it continues through 2-cell and 4-cell stages, very strongly. Hybridization signal remains fairly strong during subsequent development, including the cell proliferation and morphogenesis. | ||
| Picture | WBPicture0000011716 | ||
| Remark | Several control experiments were conducted to ascertain the specificity of the klp-17 hybridization signal. Removing the klp-17 probe, and using only the bluescript vector as a probe does not stain the nuclei. On the other hand, when klp-3 cDNA, encoding for a different C-terminus kinesin was used as a probe, the hybridization signal was observed in the entire cytoplasm, with little or no signal seen in the cell nucleus. | ||
| Reference | WBPaper00003893 |
