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WBPicture0000011716 | Description | FIG. 3. In vivo function of klp-17. (A) In situ RNA hybridization pattern of klp-17 and its comparison with klp-3, (a) one cell stage embryo which expresses in the nucleus of the cell. Similarly, (b) two cell, (c) four cell, (d) multiple cell, and (e) a young larva shows expression in the nuclei of the cell. Nuclei are indicating by arrows. In comparison, klp-3 (f-j) shows heavy staining during the entire embryogenesis. Scale bar represents 8 micron. (B) RNA interference of klp-17. (a) A wild type 1-cell embryo Nomaraski picture. (b) The same embryo as shown in a, stained with the DNA-staining dye DAPI, showing normal chromosomes in one cell stage embryo. (c) The Nomaraski image of a 2-cell stage wild type embryo. (d) The same embryo as shown in c, stained with the DAPI, showing normal chromosome. (e) Two klp-17 dsRNA treated 1-cell stage arrested embryos, Nomarski image. (f) The embryo shown in e, stained with the DNA-staining dye DAPI, showing polyploid chromosomes indicating by arrows. (g) A Nomaraski image of a 2-cell stage klp-17 dsRNA-treated arrested embryo. (j) Same embryo as shown in g, stained with the DAPI nuclear dye, showing polyploid chromosomes (indicating by arrows) in the arrested embryo. Scale bar represents 8 um. | |||
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Name | FigE.jpg | ||||
Depict | Expr_pattern | Expr982 | |||
Cellular_component | GO:0005634 | ||||
Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
Publication_year | 2000 | ||||
Article_URL | DOI | id | 10.1006/bbrc.1999.1866 | ||
Journal_URL | BiochemicalandBiophysicalResearchCommunications | ||||
Publisher_URL | Elsevier | ||||
Reference | WBPaper00003893 |