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WormBase Tree Display for Expr_pattern: Expr9494

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Name Class

Expr9494Expression_ofGeneWBGene00001018
Reflects_endogenous_expression_ofWBGene00001018
Expression_dataLife_stageWBls:0000041
WBls:0000023
Anatomy_termWBbt:0003679Certain
WBbt:0003681Certain
WBbt:0005741Uncertain
RemarkExpressed in unidentified cells in the body and tail.
WBbt:0005753Certain
WBbt:0005772Certain
WBbt:0005813Certain
WBbt:0005829Certain
WBbt:0006749Certain
WBbt:0006759Certain
GO_termGO:0005634
GO:0005737
Subcellular_localizationSub-cellular localization within the body wall muscle: Nucleus and Cytoplasm/Other
This dynamic dynactin accumulation was confirmed by immunofluorescence staining with anti-DNC-1 antibody.
When examined at the four-cell stage during P2 and EMS spindle rotation, DNC-1::GFP, DNC-2::GFP and ARP-1::GFP were all enriched at the cell border between P2 and EMS prior to and during EMS and P2 spindle rotation. The accumulation first appeared at 8.88+/-0.63 minutes after the first appearance of the cleavage furrow in P1 (n=5, measured with DNC-2::GFP embryos) and remained there for 6.8+/-0.7 minutes (n=5). Dynactin-labeled EMS and P2 centrosomes rotated and moved such that one centrosome from each spindle became closely apposed to the accumulation site.Authors also observed instances of dynactin-labeled putative microtubule (MT) plus-ends emanating from one centrosome and terminating at this accumulation site. The accumulation was then observed to disappear about 4.77+/-0.78 minutes (n=5) before EMS cleaved.
TypeReporter_gene
PatternAdult Expression: pharynx; unidentified cells in body ;unidentified cells in tail.
Adult Expression: pharynx; unidentified cells in body; unidentified cells in tail.
Larval Expression: unidentified cells in body ;unidentified cells in tail ;
Larval Expression: unidentified cells in body; unidentified cells in tail;
ReferenceWBPaper00038444
TransgeneWBTransgene00014419