WormBase Tree Display for Expr_pattern: Expr9494
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Expr9494 | Expression_of | Gene | WBGene00001018 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00001018 | ||
Expression_data | Life_stage | WBls:0000041 | |
WBls:0000023 | |||
Anatomy_term (9) | |||
GO_term | GO:0005634 | ||
GO:0005737 | |||
Subcellular_localization | Sub-cellular localization within the body wall muscle: Nucleus and Cytoplasm/Other | ||
This dynamic dynactin accumulation was confirmed by immunofluorescence staining with anti-DNC-1 antibody. | |||
When examined at the four-cell stage during P2 and EMS spindle rotation, DNC-1::GFP, DNC-2::GFP and ARP-1::GFP were all enriched at the cell border between P2 and EMS prior to and during EMS and P2 spindle rotation. The accumulation first appeared at 8.88+/-0.63 minutes after the first appearance of the cleavage furrow in P1 (n=5, measured with DNC-2::GFP embryos) and remained there for 6.8+/-0.7 minutes (n=5). Dynactin-labeled EMS and P2 centrosomes rotated and moved such that one centrosome from each spindle became closely apposed to the accumulation site.Authors also observed instances of dynactin-labeled putative microtubule (MT) plus-ends emanating from one centrosome and terminating at this accumulation site. The accumulation was then observed to disappear about 4.77+/-0.78 minutes (n=5) before EMS cleaved. | |||
Type | Reporter_gene | ||
Pattern | Adult Expression: pharynx; unidentified cells in body ;unidentified cells in tail. | ||
Adult Expression: pharynx; unidentified cells in body; unidentified cells in tail. | |||
Larval Expression: unidentified cells in body ;unidentified cells in tail ; | |||
Larval Expression: unidentified cells in body; unidentified cells in tail; | |||
Reference | WBPaper00038444 | ||
Transgene | WBTransgene00014419 |