WormBase Tree Display for Expr_pattern: Expr8987
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| Expr8987 | Expression_of | Gene | WBGene00006439 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006439 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0003679 | Partial | ||
| WBbt:0005733 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0006748 | Certain | |||
| GO_term | GO:0005739 | |||
| Subcellular_localization | In embryos, WAN-1::GFP exhibited a punctate, cytoplasmic staining pattern which overlapped with that of the mitochondrion-specific fluorescent dye, Mitotracker Red, indicating that WAN-1 is localized to mitochondria. To confirm this, WAN-1Flag was expressed in HEK293 cells. It also displayed a punctate staining pattern that completely overlapped with that of Mitotracker Red. A further subcellular fractionation analysis revealed that WAN-1Flag existed in the same fraction with that of cytochrome c. These results indicate that WAN-1 indeed localizes to mitochondria. | |||
| Type | Reporter_gene | |||
| Pattern | WAN-1::GFP expression was observed from embryonic to adult stages. In adult worms, WAN-1::GFP was seen in a wide range of tissues including the intestines, hypoderm, vulva, and some neurons. | |||
| Remark | Picture: Fig 5A, 5B, Fig S3B, S3C. | |||
| wan-1 = T27E9.1 in this paper. | ||||
| Reference | WBPaper00033159 | |||
| Transgene | WBTransgene00030844 |
