WormBase Tree Display for Expr_pattern: Expr8987
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Expr8987 | Expression_of | Gene | WBGene00006439 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00006439 | ||
Expression_data (3) | |||
Subcellular_localization | In embryos, WAN-1::GFP exhibited a punctate, cytoplasmic staining pattern which overlapped with that of the mitochondrion-specific fluorescent dye, Mitotracker Red, indicating that WAN-1 is localized to mitochondria. To confirm this, WAN-1Flag was expressed in HEK293 cells. It also displayed a punctate staining pattern that completely overlapped with that of Mitotracker Red. A further subcellular fractionation analysis revealed that WAN-1Flag existed in the same fraction with that of cytochrome c. These results indicate that WAN-1 indeed localizes to mitochondria. | ||
Type | Reporter_gene | ||
Pattern | WAN-1::GFP expression was observed from embryonic to adult stages. In adult worms, WAN-1::GFP was seen in a wide range of tissues including the intestines, hypoderm, vulva, and some neurons. | ||
Remark | Picture: Fig 5A, 5B, Fig S3B, S3C. | ||
wan-1 = T27E9.1 in this paper. | |||
Reference | WBPaper00033159 | ||
Transgene | WBTransgene00030844 |