WormBase Tree Display for Expr_pattern: Expr8987
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| Expr8987 | Expression_of (2) | |
|---|---|---|
| Expression_data (3) | ||
| Subcellular_localization | In embryos, WAN-1::GFP exhibited a punctate, cytoplasmic staining pattern which overlapped with that of the mitochondrion-specific fluorescent dye, Mitotracker Red, indicating that WAN-1 is localized to mitochondria. To confirm this, WAN-1Flag was expressed in HEK293 cells. It also displayed a punctate staining pattern that completely overlapped with that of Mitotracker Red. A further subcellular fractionation analysis revealed that WAN-1Flag existed in the same fraction with that of cytochrome c. These results indicate that WAN-1 indeed localizes to mitochondria. | |
| Type | Reporter_gene | |
| Pattern | WAN-1::GFP expression was observed from embryonic to adult stages. In adult worms, WAN-1::GFP was seen in a wide range of tissues including the intestines, hypoderm, vulva, and some neurons. | |
| Remark (2) | ||
| Reference | WBPaper00033159 | |
| Transgene | WBTransgene00030844 |
