WormBase Tree Display for Expr_pattern: Expr2857
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| Expr2857 | Expression_of | Gene | WBGene00002053 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002053 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0003673 | Certain | ||
| WBbt:0005451 | Certain | |||
| WBbt:0005812 | Certain | |||
| WBbt:0006784 | Certain | |||
| GO_term | GO:0009986 | |||
| Subcellular_localization | In embryos after the 2-fold stage, larvae, and adults, IFB-1ADGFP localized to circumferential stripes in the parts of the epidermis that contact body wall muscles and in double tracks corresponding to epidermis overlying the processes of mechanosensory neurons; these patterns of subcellular localization correspond to epidermal attachment structures, also known as fibrous organelles. IFB-1BDGFP was also expressed in the epidermis in a pattern indistinguishable from that of IFB-1A. Thus, both isoforms of IFB-1 are expressed in the epidermis and localized to the same subcellular compartments. | |||
| The monoclonal antibody MH4 recognizes an epitope common to IFA-1, IFA-2, and IFA-3, and does not recognize IFB-1. MH4 staining and IFB-1ADGFP expression co-localized in both embryonic and adult epidermal cells. In the adult epidermis, IFB-1ADGFP partly co-localized with Myotactin, a transmembrane protein that localizes to or close to the basal parts of epidermal attachments. | ||||
| Type | Reporter_gene | IFB-1BDGFP data were collected using the pCZ482 extrachromosomal array juEx595. | ||
| Pattern | IFB-1ADGFP was first detected in epidermal cells at the enclosure stage of embryogenesis. By the 1.5-fold stage, IFB-1ADGFP began to accumulate in regions of epidermal cells adjacent to body wall muscles. | |||
| In addition to epidermal and pharyngeal expression, both IFB-1 isoforms were expressed in other cell types. Both isoforms were localized to the processes of the excretory cell and the excretory duct. IFB-1B, but not IFB-1A, transgenes were highly expressed in the uterine epithelium. | ||||
| Prominent expression of IFB-1 isoforms was also observed in the pharynx. Both IFB-1A and IFB-1B are expressed in marginal cells of the pharynx. IFB-1BDGFP transgenes were expressed throughout the length of the pharynx, whereas IFB-1ADGFP transgenes were expressed in a subset of pharyngeal marginal cells. In pharyngeal marginal and muscle cells, Myotactin is localized basally, whereas IFB-1ADGFP and IFB-1BDGFP were distributed throughout the apicalbasal axis. These data confirm previous reports of anti-IFB-1 staining in pharyngeal marginal cells. | ||||
| Reference | WBPaper00006428 | |||
| Transgene | WBTransgene00000742 | |||
| WBTransgene00000705 | ||||
| WBTransgene00000741 |
