WormBase Tree Display for Transgene: WBTransgene00000705
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| WBTransgene00000705 | Public_name | juEx595 | |
|---|---|---|---|
| Summary | [IFB-1B::GFP + pRF4] | ||
| Synonym | [ifb-1B::gfp] | ||
| Construction | Construct | WBCnstr00000701 | |
| Coinjection | WBCnstr00004720 | ||
| Construction_summary | To construct GFP-tagged IFB-1B, an NcoIBamHI fragment of pCZ451, containing the 5365-bp upstream region and part of exon 1 of IFB-1B, was first cloned into the IFB-1B cDNA clone yk336b8, to make the IFB-1B minigene pCZ481. The 6.4-kb SacII fragment of pCZ481 was cloned into the SacII site of pCZ454, to create the IFB-1BDGFP minigene pCZ482. Both constructs were injected at 0.1 ng/ul with pRF4 and four transgenic lines were obtained for each. 0/4 pCZ481 lines rescued ju71. | ||
| Genetic_information | Extrachromosomal | ||
| Used_for | Expr_pattern | Expr2857 | |
| Expr12515 | |||
| Expr15156 | |||
| Associated_with | Strain | WBStrain00005392 | |
| Reference | WBPaper00006428 | ||
| Species | Caenorhabditis elegans |
