WormBase Tree Display for Expr_pattern: Expr2607
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| Expr2607 | Expression_of | Gene | WBGene00000429 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000429 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| WBls:0000015 | |||
| WBls:0000021 | |||
| WBls:0000010 | |||
| WBls:0000013 | |||
| Anatomy_term (15) | |||
| GO_term | GO:0005634 | ||
| Subcellular_localization | Antibody staining is nuclear as expected for a transcription factor. | ||
| Type | Antibody | Rabbit polyclonal peptide antibody. A synthetic peptide C-terminal to the homeodomain of CEH-2 (LEGSDPNAPMSNDEDDE) was synthesized and coupled to BSA with glutaraldehyde. Coupled antigen was injected into rabbits using RIBI adjuvants, and antisera were affinity purified against peptide-coupled BSA coupled to CNBr-activated sepharose. | |
| Pattern | Earliest expression with the antibody was seen in a small cluster in late-gastrulation embryos. From their position, these cells may be the precursors of the pharyngeal cells that express GFP in the L1 larva. ceh-2 expression was found restricted to eleven cells (fourteen nuclei) of five types in the anterior pharynx (corpus) of larvae and adults: the I3 neuron that lies embedded in the dorsal sector of the pharynx muscle; the pairs of NSM and M3 motoneurons in the left and right subventral sectors; the three m2 muscle cells, each possessing two nuclei resulting from cell fusion during development; and the three e2 epithelial cells with the anterior-most pharynx nuclei. | ||
| Picture | WBPicture0000007550 | ||
| Reference | WBPaper00005973 | ||
| Antibody_info | WBAntibody00000654 |
