WormBase Tree Display for Expr_pattern: Expr2607
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Expr2607 | Expression_of (2) | ||
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Expression_data (3) | |||
Subcellular_localization | Antibody staining is nuclear as expected for a transcription factor. | ||
Type | Antibody | Rabbit polyclonal peptide antibody. A synthetic peptide C-terminal to the homeodomain of CEH-2 (LEGSDPNAPMSNDEDDE) was synthesized and coupled to BSA with glutaraldehyde. Coupled antigen was injected into rabbits using RIBI adjuvants, and antisera were affinity purified against peptide-coupled BSA coupled to CNBr-activated sepharose. | |
Pattern | Earliest expression with the antibody was seen in a small cluster in late-gastrulation embryos. From their position, these cells may be the precursors of the pharyngeal cells that express GFP in the L1 larva. ceh-2 expression was found restricted to eleven cells (fourteen nuclei) of five types in the anterior pharynx (corpus) of larvae and adults: the I3 neuron that lies embedded in the dorsal sector of the pharynx muscle; the pairs of NSM and M3 motoneurons in the left and right subventral sectors; the three m2 muscle cells, each possessing two nuclei resulting from cell fusion during development; and the three e2 epithelial cells with the anterior-most pharynx nuclei. | ||
Picture | WBPicture0000007550 | ||
Reference | WBPaper00005973 | ||
Antibody_info | WBAntibody00000654 |