WormBase Tree Display for Expr_pattern: Expr2579
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| Expr2579 | Expression_of | Gene | WBGene00004737 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004737 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| WBls:0000015 | |||
| WBls:0000004 | |||
| WBls:0000057 | |||
| Anatomy_term (79) | |||
| GO_term | GO:0005634 | ||
| Subcellular_localization | SCC-1/COH-2 seemed to localize to the chromosomes in a cell cycle-dependent manner. In interphase, SCC-1/COH-2 was seen throughout the nucleus, overlapping largely with DNA. At mitotic prophase, SCC-1/COH-2 started to separate from condensing chromosomes, and it was not detected on the chromosomes at prometaphase and metaphase. At metaphase, the SCC-1/COH-2 signal seemed as if surrounding the metaphase plate, although it was possible that a small amount of SCC-1/COH-2 was remaining on the metaphase chromosomes but escaped detection, because cohesin is reported to become detectable on metaphase chromosomes only after detergent extraction of soluble background in other metazoans. The SCC-1/COH-2 signal was then dispersed in the cytoplasm at anaphase. At telophase, the SCC-1/COH-2 protein began to reaccumulate on the chromosomes. | ||
| Type | Antibody | Affinity purified rabbit and rat polyclonal antibodies against recombinant SCC-1. The relatively unique region in the open reading frame (amino acids 118544 for SCC-1/COH-2) was polymerase chain reaction amplified from a cDNA clone and cloned into pGEX-KG vector. Glutathione S-transferase (GST) fusion protein was expressed from the resulting plasmid in Escherichia coli and used as an antigen after purification. SCC-1/COH-2specific antibodies raised in rabbit and rat were affinity purified using histidine (His)-tagged SCC-1/COH-2 proteins, respectively. Both rabbit and rat antibodies were used in immunofluorescence analysis, and they gave the same staining patterns. | |
| Pattern (3) | |||
| Picture | WBPicture0000008732 | ||
| WBPicture0000008733 | |||
| WBPicture0000008734 | |||
| Reference | WBPaper00005937 | ||
| Antibody_info | WBAntibody00000645 | ||
| WBAntibody00000646 |
