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| WBPicture0000008732 | Description | Figure 3. Cell cycle-dependent localization of SCC-1/COH-2 in early embryos. (A) Wild-type and scc-1/coh-2(RNAi) embryos were stained with anti-SCC-1/COH-2 antibodies (SCC-1/COH-2, red) and Sytox Green (DNA, green). The white arrowheads indicate anaphase cells, in which SCC-1/ COH-2 is dissociated from the chromosomes and dispersed in the whole cytoplasm. The SCC-1/ COH-2 signal was not detected in scc-1/coh-2(RNAi) embryos. Bar, 10 um. (B) Magnified images of nuclei in wild-type early embryos. SCC-1/COH-2 accumulated in the interphase nuclei but was not detectable on condensed metaphase chromosomes. SCC-1/ COH-2 associated again with chromosomes at telophase. (C) Wild-type nuclei in an early embryo were stained with anti-SCC- 1/COH-2 antibodies (SCC-1/ COH-2, red), an anti-nuclear pore complexes antibody (NPCs, green), and 4,6-diamidino-2-phenylindole (DNA). It is noticeable that few SCC-1/COH-2 molecules associate chromosomes at metaphase but they stay in the nucleoplasm surrounded by the nuclear membrane. | |||
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| Name | F3.large.jpg | ||||
| Depict | Expr_pattern | Expr2579 | |||
| Cellular_component | GO:0005634 | ||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1091/mbc.E02-09-0603 | ||
| Journal_URL | MolecularBiologyoftheCell | ||||
| Publisher_URL | TheAmericanSocietyForCellBiology | ||||
| Reference | WBPaper00005937 |
