WormBase Tree Display for Expr_pattern: Expr2459
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| Expr2459 | Expression_of | Gene | WBGene00004726 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004726 | ||||
| Expression_data | Life_stage | WBls:0000003 | |||
| WBls:0000041 | |||||
| Anatomy_term | WBbt:0005175 | Partial | |||
| Remark | Expressed in the syncytial part of the gonad. | ||||
| WBbt:0006798 | Certain | ||||
| GO_term | GO:0005813 | ||||
| Subcellular_localization | In newly fertilized embryos, SAS-4 is localized to a discrete spot near the sperm-derived pronucleus. At a slightly later stage, gamma-tubulin is recruited to the sperm centrioles forming a focus that colocalizes with SAS-4. As the embryo proceeds into mitosis, the amount of gamma-tubulin at centrosomes dramatically increases. In contrast, the SAS-4 staining remains unchanged as a small dot in the center of the centrosome. | ||||
| Type | Antibody | Immunofluorescence of whole worms and fixed embryos. Affinity-purified polyclonal rabbit antibody against SAS-4 recombinant protein. 6xHis tagged full-length SAS-4 was prepared by amplifying the cDNA, yk425b11, using the following primers: CGCGCGGGATCCGCTTCCGATGAAAATATCGGTG and CGCGCGCTCGAGTCATTTTTTCCACTGGAACAAAGTT, digesting the product with BamHI/XhoI and cloning into pRSET-A (Invitrogen). Antibodies to the fusion protein were generated in rabbits and affinity purified. | |||
| Pattern | In worms, SAS-4 colocalized with gamma-tubulin to centrosomes both in sperm and in the syncytial part of the gonad. SAS-4 staining in the gonad disappeared as the meiotic nuclei cellularized to form oocytes, presumably marking the point at which the centrioles are lost during oogenesis. | ||||
| Reference | WBPaper00005739 | ||||
| Antibody_info | WBAntibody00000606 |
