WormBase Tree Display for Expr_pattern: Expr1926
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| Expr1926 | Expression_of | Gene | WBGene00001852 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001852 | ||||
| Expression_data | Life_stage | WBls:0000024 | |||
| WBls:0000038 | |||||
| WBls:0000027 | |||||
| WBls:0000035 | |||||
| WBls:0000041 | |||||
| WBls:0000015 | |||||
| WBls:0000021 | |||||
| WBls:0000010 | |||||
| WBls:0000013 | |||||
| Anatomy_term | WBbt:0003673 | Certain | |||
| WBbt:0005736 | Certain | ||||
| WBbt:0005812 | Certain | ||||
| WBbt:0005813 | Certain | ||||
| WBbt:0005821 | Certain | ||||
| WBbt:0006751 | Certain | ||||
| Remark | Expressed in a limited number of head neurons. | ||||
| GO_term | GO:0005730 | ||||
| GO:0005634 | |||||
| GO:0005737 | |||||
| GO:0000793 | |||||
| GO:0045111 | |||||
| Subcellular_localization | A double labeling experiment was performed with H1.X-11 and the monoclonal antibody IFA, which stains the intermediate filament proteins of the tonofilaments. Both antibodies label identical structures - the tonofilaments in the marginal cells. | ||||
| Antibody labeling with H1.X-101 revealed a prominent labeling of the nucleoli in the polyploid gut nuclei of adult C. elegans hermaphrodites, which colocalized with fibrillarin, a structural component of small nucleolar RNPs (snoRNPs). H1.X was never detected in condensed mitotic or meiotic chromosomes or as a structural component of the interphase chromatin, as revealed by antibody labeling ( H1.X-11) of embryos and meiotic oocytes. H1.X::GFP was never found localized to condensed chromosomes. H1.1-GFP fluorescence is readily observed in condensed mitotic chromosomes in C. elegans embryos, and it is detected as a structural component of interphase chromatin in antibody labeling of embryonic blastomeres and all other cell types of C. elegans. | |||||
| H1.X-101 did not label the nuclear lamina. In H1.X::gfp transgenic animals, the cytoplasm of the marginal cells appeared brightly fluorescent, and the nuclei therein became visible as the brightest fluorescent structures of the cells. The GFP fluorescence was almost homogeneously distributed throughout the whole cytoplasm, but the tonofilaments therein became clearly visible as bright green dots when visualized in axial orientation. | |||||
| Type | Antibody | Polyclonal rabbit antibody against H1.X. | |||
| Reporter_gene | |||||
| Pattern | H1.X::GFP is expressed in body-wall muscles, as well as in the vulva sex muscles. In both cases the general appearance of the cells corresponds to the situation already described for the marginal cells of the pharynx: the fluorescence signal fills the cytoplasm, and the cells' nuclei appear as the brightest fluorescent structure. H1.X presence in muscle cells was not detected with the two different antibodies. H1.X::GFP was also expressed in a limited number of head neurons, in which the fluorescence signal filled the total cytoplasm, including the neuronal projections. The presence of H1.X in these cells was confirmed with the H1.X-101 antibody. Furthermore, H1.X::GFP was detected in the cytoplasm of excretory cells. | ||||
| In C. elegans embryos, H1.X::GFP expression starts with the 30-cell stage. The most prominent structures labeled with both antibodies as well as with H1.X::GFP in transgenic animals are the marginal cells and the tonofilaments therein. The signal appears in all larval stages and in adult C. elegans. No further nuclear or cytoplasmic signal appears besides the labeling of all nuclear lamina caused by the anti-lamin crossreactivity of H1.X-11. This result is confirmed by independent antibody labeling with H1.X-101. In these preparations no nuclear signal appeared in the nuclei of the marginal cells, although the cytoplasm of some head neurons was stained. | |||||
| Picture | WBPicture0000009033 | ||||
| WBPicture0000009034 | |||||
| WBPicture0000009035 | |||||
| WBPicture0000009036 | |||||
| WBPicture0000009038 | |||||
| Remark | hil-1 = C30G7.1 = H1.X | ||||
| Reference | WBPaper00005366 | ||||
| Transgene | WBTransgene00027756 | ||||
| Antibody_info | WBAntibody00000532 | ||||
| WBAntibody00000533 |
