WormBase Tree Display for Expr_pattern: Expr1926
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Expr1926 | Expression_of | Gene | WBGene00001852 | ||
---|---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00001852 | ||||
Expression_data | Life_stage | WBls:0000024 | |||
WBls:0000038 | |||||
WBls:0000027 | |||||
WBls:0000035 | |||||
WBls:0000041 | |||||
WBls:0000015 | |||||
WBls:0000021 | |||||
WBls:0000010 | |||||
WBls:0000013 | |||||
Anatomy_term | WBbt:0003673 | Certain | |||
WBbt:0005736 | Certain | ||||
WBbt:0005812 | Certain | ||||
WBbt:0005813 | Certain | ||||
WBbt:0005821 | Certain | ||||
WBbt:0006751 | Certain | ||||
Remark | Expressed in a limited number of head neurons. | ||||
GO_term | GO:0005730 | ||||
GO:0005634 | |||||
GO:0005737 | |||||
GO:0000793 | |||||
GO:0045111 | |||||
Subcellular_localization (3) | |||||
Type | Antibody | Polyclonal rabbit antibody against H1.X. | |||
Reporter_gene | |||||
Pattern | H1.X::GFP is expressed in body-wall muscles, as well as in the vulva sex muscles. In both cases the general appearance of the cells corresponds to the situation already described for the marginal cells of the pharynx: the fluorescence signal fills the cytoplasm, and the cells' nuclei appear as the brightest fluorescent structure. H1.X presence in muscle cells was not detected with the two different antibodies. H1.X::GFP was also expressed in a limited number of head neurons, in which the fluorescence signal filled the total cytoplasm, including the neuronal projections. The presence of H1.X in these cells was confirmed with the H1.X-101 antibody. Furthermore, H1.X::GFP was detected in the cytoplasm of excretory cells. | ||||
In C. elegans embryos, H1.X::GFP expression starts with the 30-cell stage. The most prominent structures labeled with both antibodies as well as with H1.X::GFP in transgenic animals are the marginal cells and the tonofilaments therein. The signal appears in all larval stages and in adult C. elegans. No further nuclear or cytoplasmic signal appears besides the labeling of all nuclear lamina caused by the anti-lamin crossreactivity of H1.X-11. This result is confirmed by independent antibody labeling with H1.X-101. In these preparations no nuclear signal appeared in the nuclei of the marginal cells, although the cytoplasm of some head neurons was stained. | |||||
Picture | WBPicture0000009033 | ||||
WBPicture0000009034 | |||||
WBPicture0000009035 | |||||
WBPicture0000009036 | |||||
WBPicture0000009038 | |||||
Remark | hil-1 = C30G7.1 = H1.X | ||||
Reference | WBPaper00005366 | ||||
Transgene | WBTransgene00027756 | ||||
Antibody_info | WBAntibody00000532 | ||||
WBAntibody00000533 |