WormBase Tree Display for Expr_pattern: Expr1858
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| Expr1858 | Expression_of | Gene | WBGene00004874 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004874 | ||
| Expression_data | GO_term | GO:0000793 | |
| GO:0000775 | |||
| Subcellular_localization | SMC-4 antibody staining showed that SMC-4 localized to mitotic chromosomes in a dynamic pattern with two striking features. First, SMC-4 localized to chromosomes only at times of the cell cycle when they were condensed. Little or no SMC-4 was observed on decondensed chromosomes during interphase. SMC-4 localized to chromosomes at prometaphase, when the six C. elegans chromosomes, but not their sister chromatids, become visibly distinct. SMC-4 persisted through metaphase and anaphase, then disappeared again as chromosomes decondensed and entered telophase. Second, SMC-4 associated only with certain regions of the chromosomes. SMC-4 appeared in a faint speckled pattern during prophase, then appeared in two stripes outlining each chromosome during prometaphase. When chromosomes were aligned at metaphase, SMC-4 localized to distinct foci on the poleward sides of the metaphase plate. The foci appear as paired spots of staining in cross-section and may represent the poleward sides of paired sister chromatids. | ||
| Type | Antibody | SMC-4 Rabbit and rat polyclonal antibodies were made to fusion proteins covering amino-terminal or central epitopes and to amino- or carboxy-terminal peptides. | |
| Picture | WBPicture0000008428 | ||
| WBPicture0000008430 | |||
| WBPicture0000008432 | |||
| WBPicture0000008434 | |||
| Remark | No detailed description on cellular expression patterns. | ||
| Reference | WBPaper00005173 | ||
| Antibody_info | WBAntibody00000481 | ||
| WBAntibody00000482 | |||
| WBAntibody00000483 | |||
| WBAntibody00000484 |
