WormBase Tree Display for Picture: WBPicture0000008428

WBPicture0000008428 Description: Figure 2. SMC-4 colocalizes with MIX-1 at the poleward face of condensed mitotic chromosomes but not on X chromosomes. (A-E) The mitotic chromosome condensation cycle. Merged confocal images of wild-type embryonic nuclei costained with DNA dye (red) and tubulin antibody (green) show C. eleganschromosomes and microtubules throughout mitosis. (F-J) Merged confocal images of wild-type embryonic nuclei costained with SMC-4 antibody (green) and DNA dye (red); overlap appears yellow. (K-N) Early wild-type embryo costained with DNA dye (blue, K), MIX-1 antibody (red,L), and SMC-4 antibody (green, M). MIX-1 and SMC-4 colocalize in nuclei with condensed chromosomes (yellow when merged,N). At this early stage, dosage compensation is not active; MIX-1 and other dosage compensation proteins are not localized to the X chromosome. (O-Q) Metaphase chromosomes costained with DNA dye (blue), MIX-1 antibody (red, O), and SMC-4 antibody (green, P) show colocalization of MIX-1 and SMC-4 at the poleward face of condensed chromosomes (yellow when merged,Q). (R-T) Wild-type larva costained for DNA (blue), MIX-1 (red), and SMC-4 (green). MIX-1 and SMC-4 both stain nuclei of two mitotic cells (mit), but only MIX-1 shows the punctate subnuclear staining characteristic of dosage compensation proteins bound to hermaphrodite X chromosomes (X).
  Name: F2.large.jpg
  Depict: Expr_pattern: Expr1858
      Expr1859
    Cellular_component: GO:0000793
  Acknowledgment: Template: WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2002
    Article_URL: DOI id 10.1101/gad.968302
    Journal_URL: Genes&Development
    Publisher_URL: ColdSpringHarborLabPress
  Reference: WBPaper00005173