WormBase Tree Display for Expr_pattern: Expr1737
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| Expr1737 | Expression_of | Gene | WBGene00004782 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004782 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0004017 | Certain | |||
| WBbt:0004575 | Certain | |||
| WBbt:0004576 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0005784 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | nuclei | |||
| Type | Antibody | Rat polyclonal antibodies were raised against the N-terminal peptide of SET-2L. The distribution of SET-2L in wild-type worms and embryos was determined by immunostaining. | ||
| Pattern | SET-2L protein is localized in the nuclei of all cells in embryos at all stages of embryogenesis. In L1 larvae, SET-2L remains visible in the nuclei of most cell types but is most prominent in Z2 and Z3, the primordial germ cells. In adults, SET-2L staining is nuclear and is strongest in the germline, although it is also detectable in other cell types, such as intestinal, pharyngeal, and neuronal cells. The level of SET-2L varies in different portions of the gonad. It is present in the distal mitotic cells, diminishes to very low levels in the transition zone and early pachytene regions, and increases dramatically in mid-pachytene nuclei. In oocytes, the level of SET-2L appears relatively low. | |||
| Picture | WBPicture0000011763 | |||
| WBPicture0000011764 | ||||
| Remark | The specificity of the antibodies was demonstrated by the observation that SET-2L immunostaining is reduced to below detection in set-2(RNAi) embryos and worms. | |||
| Ubiquitous. The specificity of the antibodies was demonstrated by the observation that SET-2L immunostaining is reduced to below detection in set-2(RNAi) embryos and worms. | ||||
| Reference | WBPaper00005020 | |||
| Antibody_info | WBAntibody00000438 |
