WormBase Tree Display for Expr_pattern: Expr11256

Expr11256 Expression_of: Gene: WBGene00004140
    Reflects_endogenous_expression_of: WBGene00004140
  Expression_data: Life_stage: WBls:0000003
      WBls:0000023
    Anatomy_term: WBbt:0004757 Certain
      WBbt:0004758 Certain
      WBbt:0005735 Certain
      WBbt:0005829 Certain
      WBbt:0006749 Certain
      WBbt:0006759 Certain
    GO_term: GO:0030424
      GO:0043025
      GO:0005829
  Subcellular_localization: EBAX-1::GFP showed a punctate pattern in the cytosol of individual neurons. A similar punctate pattern was also observed in the soma and axons when EBAX-1::GFP was specifically expressed in mechanosensory neurons and GABAergic motor neurons (data not shown). Likewise, GFP-tagged mouse ZSWIM8 displayed a cytosolic expression pattern in cultured heterologous cells.
  Type: Reporter_gene
  Pattern: In C. elegans, ebax-1 transcriptional and translational reporters showed that EBAX-1 is enriched in the developing nervous system. A functional C-terminal GFP-fusion of EBAX-1 (EBAX- 1::GFP) driven by the endogenous 2.7 kb promoter showed dynamic expression throughout embryonic and larval stages. Fluorescence was detected from midembryogenesis, with a higher level in the anterior half of the embryo. The expression at the far posterior end increased at the 2-fold stage. The overall intensity of EBAX-1::GFP peaked at the 3-fold stage, especially around the nerve ring region and dropped after hatching. In the fourth larval stage (L4), EBAX-1::GFP was detected in the nerve ring, the ventral nerve cord, the HSN motor neuron, and some neurons in the tail. Mos1 transposase-mediated single copy insertion (MosSCI) of Pebax- 1::EBAX-1::GFP showed similar expression dynamics, albeit at a lower expression level (data not shown).
  Picture: WBPicture0000013469
    WBPicture0000013471
  Reference: WBPaper00044158
  Transgene: WBTransgene00020055