WormBase Tree Display for Expr_pattern: Expr11256
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Expr11256 | Expression_of (2) | |
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Expression_data (3) | ||
Subcellular_localization | EBAX-1::GFP showed a punctate pattern in the cytosol of individual neurons. A similar punctate pattern was also observed in the soma and axons when EBAX-1::GFP was specifically expressed in mechanosensory neurons and GABAergic motor neurons (data not shown). Likewise, GFP-tagged mouse ZSWIM8 displayed a cytosolic expression pattern in cultured heterologous cells. | |
Type | Reporter_gene | |
Pattern | In C. elegans, ebax-1 transcriptional and translational reporters showed that EBAX-1 is enriched in the developing nervous system. A functional C-terminal GFP-fusion of EBAX-1 (EBAX- 1::GFP) driven by the endogenous 2.7 kb promoter showed dynamic expression throughout embryonic and larval stages. Fluorescence was detected from midembryogenesis, with a higher level in the anterior half of the embryo. The expression at the far posterior end increased at the 2-fold stage. The overall intensity of EBAX-1::GFP peaked at the 3-fold stage, especially around the nerve ring region and dropped after hatching. In the fourth larval stage (L4), EBAX-1::GFP was detected in the nerve ring, the ventral nerve cord, the HSN motor neuron, and some neurons in the tail. Mos1 transposase-mediated single copy insertion (MosSCI) of Pebax- 1::EBAX-1::GFP showed similar expression dynamics, albeit at a lower expression level (data not shown). | |
Picture (2) | ||
Reference | WBPaper00044158 | |
Transgene | WBTransgene00020055 |