WormBase Tree Display for Variation: WBVar00241162
expand all nodes | collapse all nodes | view schema
WBVar00241162 | Evidence | Paper_evidence | WBPaper00026839 | ||||||
---|---|---|---|---|---|---|---|---|---|
Name | Public_name | q626 | |||||||
Other_name | CE21610:p.Ala205Thr | ||||||||
Y38F1A.5.1:c.613G>A | |||||||||
Y38F1A.5.2:c.613G>A | |||||||||
HGVSg | CHROMOSOME_II:g.12989241G>A | ||||||||
Sequence_details | SMap | S_parent | Sequence | Y38F1A | |||||
Flanking_sequences | acacttcaatgggagaccgagtcgccgacg | cgttctccttcttcgactttctcgcctcaa | |||||||
Mapping_target | Y38F1A | ||||||||
Type_of_mutation | Substitution | g | a | Paper_evidence | WBPaper00026839 | ||||
SeqStatus | Sequenced | ||||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Strain | WBStrain00047322 | ||||||||
Laboratory | JK | ||||||||
Status | Live | ||||||||
Affects | Gene | WBGene00000870 | |||||||
Transcript | Y38F1A.5.2 | VEP_consequence | missense_variant | ||||||
VEP_impact | MODERATE | ||||||||
SIFT | 0.03 | deleterious | |||||||
PolyPhen | 0.996 | probably_damaging | |||||||
HGVSc | Y38F1A.5.2:c.613G>A | ||||||||
HGVSp | CE21610:p.Ala205Thr | ||||||||
cDNA_position | 844 | ||||||||
CDS_position | 613 | ||||||||
Protein_position | 205 | ||||||||
Exon_number | 5/8 | ||||||||
Codon_change | Gcg/Acg | ||||||||
Amino_acid_change | A/T | ||||||||
Y38F1A.5.1 | VEP_consequence | missense_variant | |||||||
VEP_impact | MODERATE | ||||||||
SIFT | 0.03 | deleterious | |||||||
PolyPhen | 0.996 | probably_damaging | |||||||
HGVSc | Y38F1A.5.1:c.613G>A | ||||||||
HGVSp | CE21610:p.Ala205Thr | ||||||||
cDNA_position | 613 | ||||||||
CDS_position | 613 | ||||||||
Protein_position | 205 | ||||||||
Exon_number | 3/6 | ||||||||
Codon_change | Gcg/Acg | ||||||||
Amino_acid_change | A/T | ||||||||
Interactor | WBInteraction000052039 | ||||||||
WBInteraction000052040 | |||||||||
WBInteraction000052041 | |||||||||
WBInteraction000052042 | |||||||||
WBInteraction000052043 | |||||||||
WBInteraction000502228 | |||||||||
WBInteraction000502229 | |||||||||
WBInteraction000502230 | |||||||||
WBInteraction000519091 | |||||||||
WBInteraction000519093 | |||||||||
WBInteraction000538726 | |||||||||
WBInteraction000538727 | |||||||||
WBInteraction000538728 | |||||||||
WBInteraction000538730 | |||||||||
WBInteraction000538732 | |||||||||
Genetics | Interpolated_map_position | II | 13.3124 | ||||||
Description | Phenotype | WBPhenotype:0000306 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "In wild-type animals of both sexes, fkh-6::GFP expression began in the SGPs at about 6hr after hatching (Figures 6A and 6H, black bars). By contrast, fkh-6::GFP expression was either delayed or absent in the SGPs of cyd-1(q626) mutants in both sexes (Figures 6D and 6H, red bars). Specifically, few SGPs expressed fkh-6::GFP 6 hr after hatching, only about half expressed the reporter after 8 hr (males, 50%, n = 60; hermaphrodites, 43%, n = 124), and even 12 hr after hatching, fkh-6::GFP was absent from many SGPs (Figures 6D and 6H, red bars)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0007854 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Life_stage | WBls:0000024 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | ezIs2 [fkh-6::GFP] | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000318 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "We next asked if the timing of cell divisions was impaired in cyd-1(q626) mutants relative to wild-type. At the restrictive temperature, wild-type SGPs divided between 7 and 9 hr after hatching in both sexes (males,n = 9; hermaphrodites, n = 13), and two subsequent divisions in the SGP lineage occurred at approximately 10 and 13 hr after hatching, respectively (Figure 3D). By contrast, cyd-1(q626) SGPs divided 12 or more hr after hatching, just prior to or during the L1 molt, in both sexes (males, n = 7; hermaphrodites, n = 11) (Figure 3D)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0007854 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Life_stage | WBls:0000024 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000594 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Hermaphrodite SGPs produce daughters of approximately equal size (Figures 1A and 2H), and their proximal daughters do not migrate; however, male SGPs produce daughters of unequal size (Figures 1A and 2G), and their proximal daughters migrate toward the anterior. In q626 males, the SGP daughters were of roughly equal size (Figure 2I), and most proximal daughters did not migrate (92%, n = 13). We conclude that q626 feminizes both the SGP asymmetric division and later gonadal differentiation." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0007854 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000691 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "To learn when q626 affects gonadogenesis, we imposed selected temperature regimens on this temperature-sensitive mutant. In both hermaphrodites and males, the gonad developed normally when raised at the permissive temperature during the L1 stage and then shifted to the restrictive temperature from the L1 molt through adulthood; by contrast, gonad development was defective when L1 larvae were exposed to the restrictive temperature and were then returned to the permissive temperature from the L1 molt through adulthood (Figures 1I and 1J). We conclude that q626 affects gonadogenesis during the L1 stage in both sexes." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005175 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Life_stage | WBls:0000024 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001276 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "The hermaphrodite-specific marker cdh-3::GFP labels the hermaphrodite anchor cell, but it is not expressed in wild-type male gonads (Figures 2A and 2B) (Pettitt et al., 1996); however, cdh-3::GFP expression occurred in most q626 males (67%, n = 15; Figure 2C)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
"Finally, three other hermaphrodite-specific markers of gonadal differentiation were expressed in most q626 male gonads: lim-7::GFP (sheath cells), fkh-6::GFP (sheath and spermatheca), and ZK813.3::GFP (spermatheca) (data not shown). We conclude that the disorganized gonads in q626 males are feminized." | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006794 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | arIs51 [cdh-3::GFP] | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
tnIs6 [lim-7::GFP] | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
ezIs2 [fkh-6::GFP] | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
leEx780 [ZK813.3::GFP] | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001278 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "In wild-type L1 larvae, rnr::GFP expression first begins in the SGPs approximately 6 hr after hatching, and also occurs in other dividing cells (Figure 6C). By contrast, rnr::GFP was rarely expressed in cyd-1(q626) SGPs (6%, n = 32 SGPs), but it was expressed in nongonadal cells (Figure 6F)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0007854 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Life_stage | WBls:0000024 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | maIs103 [rnr::GFP] | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001355 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "We have isolated a temperature-sensitive mutation, called q626 (see Experimental Procedures). At the permissive temperature of 20C, q626 homozygotes of either sex were virtually normal, but, at the restrictive temperature of 25C, they had gonadal defects in both sexes." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005175 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001375 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "By contrast, the male-specific marker K09C8.2::GFP labels the male seminal vesicle and vas deferens, but it is not expressed in wild-type hermaphrodite gonads (Figures 2D and 2E) (Chang et al., 2004); K09C8.2::GFP was not detected in some q626 males (45%, n = 51; Figure 2F)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Incomplete | 45 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006870 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0005337 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | ezIs1 [K09C8.2::GFP] | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001585 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Hermaphrodite SGPs produce daughters of approximately equal size (Figures 1A and 2H), and their proximal daughters do not migrate; however, male SGPs produce daughters of unequal size (Figures 1A and 2G), and their proximal daughters migrate toward the anterior. In q626 males, the SGP daughters were of roughly equal size (Figure 2I), and most proximal daughters did not migrate (92%, n = 13). We conclude that q626 feminizes both the SGP asymmetric division and later gonadal differentiation." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0007854 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001656 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Whereas all wild-type male gonads had two lag-2::GFP-expressing mDTCs (100%, n = 20), only a small percentage of q626 males had detectable lag-2::GFP-expressing mDTCs (23%, n = 78). Therefore, mDTCs are not generated normally in q626 XO gonads. We conclude that q626 affects the production of distal SGP daughters in both sexes." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006864 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | qIs56 [lag-2::GFP] | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001675 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "In contrast to this relatively mild effect on hermaphrodites, q626 had a severe effect on male gonadogenesis. Wild-type adult males and most adult q626 males raised at 20C have an extended J-shaped gonad (Figures 1E and 1H). By contrast, most adult q626 males had disorganized gonads that were not extended when raised at 25C (Figures 1F and 1H)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Incomplete | 82 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006794 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | him-5(e1490) | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001929 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "At the restrictive temperature, about one-third of q626 hermaphrodites were missing one lag-2::GFP-expressing DTC (32%, n = 91, data not shown), and about one-half of q626 males were missing the lag-2::GFP-expressing LC (58%, n = 78, data not shown). Therefore, the lack of arm extension in q626 gonads is due to missing leader cells." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Incomplete | 58 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005062 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | qIs56 [lag-2::GFP] | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001962 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "At the restrictive temperature, about one-third of q626 hermaphrodites were missing one lag-2::GFP-expressing DTC (32%, n = 91, data not shown), and about one-half of q626 males were missing the lag-2::GFP-expressing LC (58%, n = 78, data not shown). Therefore, the lack of arm extension in q626 gonads is due to missing leader cells." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Incomplete | 32 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006863 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | qIs56 [lag-2::GFP] | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001968 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "We next asked if cyd-1(q626) affects POP-1 asymmetry. In wild-type animals, GFP::POP-1 is higher in the nuclei of proximal than distal SGP daughters (Figures 6B-6D; Siegfried et al., 2004). By contrast, in cyd-1(q626) hermaphrodites, GFP::POP-1 asymmetry in SGP daughters was sometimes abolished: in 4 of 12 animals, nuclear GFP::POP-1 was present at approximately equal levels in one pair of SGP daughter cells (Figures 5E-5G)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0007854 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | qIs74 [GFP::POP-1] | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0002207 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Normally, hermaphrodites have a gonad with two extended U-shaped "arms," one anterior and one posterior (Figures 1C and 1G), but when raised at 25C, some q626 hermaphrodites were missing either one or both arms (Figures 1D and 1G). Importantly, q626 hermaphrodites with one gonadal arm were self-fertile, and no other gonadal defects were observed." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Incomplete | 40 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005178 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0002457 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Furthermore, some male q626 mutants developed a hermaphrodite vulva (3%, n = 145; data not shown)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Low | 3 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006748 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0040025 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_not_observed | WBPhenotype:0000030 | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "These q626 effects were gonad specific in both sexes: growth was similar to that of wild-type animals, movement seemed unaffected, nongonadal tissues developed normally, and embryonic survival was normal." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000050 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "These q626 effects were gonad specific in both sexes: growth was similar to that of wild-type animals, movement seemed unaffected, nongonadal tissues developed normally, and embryonic survival was normal." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000306 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "The hermaphrodite spermathecal expression (of fkh-6::GFP) could not be tested in cyd-1(0) mutants, but it appeared normal in cyd-1(q626) hermaphrodites at the restrictive temperature (100%, n = 50)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
"We next examined other SGP markers. In wild-type larvae, tra-1::GFP, pes-1::GFP, and lag-2::GFP are all expressed in both hermaphrodite and male SGPs (Hope, 1991; Mathies et al., 2004) (Figure 6B, data not shown); similarly, all three reporters were expressed in SGPs of both cyd-1(q626) and cyd-1(he116) mutants in both sexes (Figure 6E; data not shown). Therefore, cyd-1 does not control gene expression in SGPs generally." | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005319 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0007854 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | ezIs2 [fkh-6::GFP] | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
qIs76 [tra-1::GFP] | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
qIs61 [pes-1::GFP] | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
qIs56 [lag-2::GFP] | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000318 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Subsequent rounds of division occurred during L2, and their timing was normal relative to the SGP division (Figure 3D)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
"Intestinal divisions were not delayed in cyd-1(q626) mutants, and the adult number of intestinal nuclei was normal (n = 20; Figure 3D)." | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0007854 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0005792 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Life_stage | WBls:0000107 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0008406 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
GO:0048565 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000530 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "These q626 effects were gonad specific in both sexes: growth was similar to that of wild-type animals, movement seemed unaffected, nongonadal tissues developed normally, and embryonic survival was normal." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000618 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "... both cyd-1(q626) hermaphrodites and males had the normal number of coelomocytes (six in hermaphrodites, five in males), which was seen when using the unc-122::GFP coelomocyte marker (data not shown)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005751 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | qIs56 [unc-122::GFP] | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000643 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "These q626 effects were gonad specific in both sexes: growth was similar to that of wild-type animals, movement seemed unaffected, nongonadal tissues developed normally, and embryonic survival was normal." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000746 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Similarly, the B blast cell divided normally in cyd-1(q626) males (n = 20; Figure 3D)..." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0003825 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001585 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "In wild-type hermaphrodites, nuclear GFP::POP-1 is more abundant in anterior T and V daughters (Herman, 2002). Similarly, POP-1 asymmetry was normal in T and V cell daughters in cyd-1(q626) mutants (n = 28)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0004946 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0004944 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0008597 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001894 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Intestinal divisions were not delayed in cyd-1(q626) mutants, and the adult number of intestinal nuclei was normal (n = 20; Figure 3D)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005772 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
GO_term | GO:0048565 | PATO:0000460 | Paper_evidence | WBPaper00026839 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0002211 | Paper_evidence | WBPaper00026839 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "We also looked for T cell lineage defects in cyd-1(q626) animals by their ability to take up the lipophilic dye, DiO (Herman and Horvitz, 1994). In wild-type animals, descendents of the T cell (phasmid neurons in the tail, PHA and PHB) stain with this dye. DiO was taken up normally by phasmid neurons in all cyd-1(q626) mutants (n = 84)." | Paper_evidence | WBPaper00026839 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005425 | PATO:0000460 | Paper_evidence | WBPaper00026839 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Reference | WBPaper00026839 | ||||||||
Method | Substitution_allele |