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WormBase Tree Display for Strain: WBStrain00037033

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Name Class

WBStrain00037033StatusLive
Genotypesnr-3&rsp-5(ok2084)/mIn1 [mIs14 dpy-10(e128)] II.
Public_nameVC2013
ContainsGeneWBGene00001072
WBGene00004702
WBGene00004916
VariationWBVar00142982
WBVar00093269
RearrangementmIn1
mIn1[dpy-10(e128) mIs14(myo-2::GFP)]
TransgeneWBTransgene00000999
PropertiesOutcrossedx2
MutagenEMS
CGC_received14 May 2008 00:00:00
LocationCGC
Made_byWBPerson154
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
T28D9.10, T28D9.2. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok2084 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: GGTTCCGCAAAGTGCATAAT. External right primer: CTAGGAAGAGCGCGAACAAC. Internal left primer: GTTGACTCGGAAAGCCGTAA. Internal right primer: AGGAAGCGGTGTCCTACTCA. Internal WT amplicon: 3125 bp. Deletion size: 1493 bp. Deletion left flank: AGTATAGGACGTTCGATACTCAAATTTGCT. Deletion right flank: CGTGATCGCAAACGTTCTCGCAGATCCCGT.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans