WormBase Tree Display for Interaction: WBInteraction000542782
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| WBInteraction000542782 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00000912 | Interactor_type | Trans_regulated | |
| WBGene00002004 | Interactor_type | Trans_regulated | |||
| WBGene00004804 | Interactor_type | Trans_regulated | |||
| WBGene00004932 | Interactor_type | Trans_regulated | |||
| WBGene00001758 | Interactor_type | Trans_regulated | |||
| WBGene00001752 | Interactor_type | Trans_regulated | |||
| Other_interactor | NT219 | Interactor_type | Trans_regulator | ||
| Interaction_summary | "Using quantitative real-time PCR (qPCR) and specific primer sets, we tested the effect of NT219 on the expression of DAF-16, HSF-1, and SKN- 1 target genes in temperature sensitive sterile worms (strain CF512)... The worms were grown on EV bacteria and treated for 3 h with 600 lM NT219 at days 1 and 2 of adulthood. An identical group of worms was treated with the vehicle (Ve), and a third group was grown on daf-2 RNAi bacteria... Three independent experiments showed that NT219 (Fig. 2D-F, spotted bars) elevates the expression levels of all genes compared with the levels seen in control worms (black bars). Yet, while DAF-16 (D) and SKN-1 (F) target genes exhibited average increase levels of 19-37%, the expression level of hsp-70 was elevated by approximately 170% (E)." | ||||
| Detection_method | RT_PCR | ||||
| Regulation_level | Transcriptional | ||||
| Regulation_result | Positive_regulate | Life_stage | WBls:0000041 | ||
| Paper | WBPaper00044508 | ||||
| Remark | Fig. 2def; NT219 (600uM) |
