WormBase Tree Display for Interaction: WBInteraction000504430
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| WBInteraction000504430 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00003043 | Interactor_type | Trans_regulated | |
| Transgene | WBTransgene00000964 | ||||
| Construct | WBCnstr00000956 | ||||
| WBGene00038147 | Interactor_type | Trans_regulator | |||
| Variation_interactor | WBVar00296476 | Interactor_type | Trans_regulator | ||
| Interaction_summary | The expression of lip-1::GFP in C. briggsae (mfIs29) was first observed in all six VPCs during the L2 stage (50%, n = 14). This pattern was dynamic, such that by the early/mid-L3 (Pn.p) stage the fluorescence could be seen in only P5.p and P7.p and was undetectable in other Pn.p cells (48%, n = 21). This indicates that while lip-1 expression in C. briggsae is maintained in 20 precursors it is rapidly downregulated in 10 and other cells. An analogous pattern was observed in mid-L3 (Pn.px) stage animals although in few cases (27%, n = 11 animals) faint GFP fluorescence was also detected in P4.p and P8.p daughters. At later stages no fluorescence was seen in P(5-7).p progeny. The analysis of lip-1 reporter expression in Cbr-pry-1(sy5353) animals revealed a similar profile but the fluorescence was much higher in P7.p and P8.p cells. Thus, in L2 stage animals all six VPCs were seen fluorescing, with P7.p and P8.p being the brightest in half of the animals (n = 6) that showed GFP fluorescence. By the early/mid-L3 (Pn.p) stage, fluorescence had rapidly faded in P6.p and other anterior VPCs, however, P7.p and P8.p continued to fluoresce brightly (90%, n = 11 GFP expressing animals). After the mid-L3 (Pn.px) stage, other than P7.p and P8.p, no VPC progeny showed detectable level of fluorescence (71%, n = 14 GFP expressing animals). | ||||
| Detection_method | Construct | ||||
| Reporter_gene | [lip-1::gfp] | ||||
| Transgene | |||||
| Regulation_level | Transcriptional | ||||
| Regulation_result | Negative_regulate | Life_stage | WBls:0000038 | ||
| Anatomy_term | WBbt:0008129 | ||||
| WBbt:0008133 | |||||
| Paper | WBPaper00036467 |
