WormBase Tree Display for Interaction: WBInteraction000501547
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| WBInteraction000501547 | Interaction_type | Regulatory | |||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00003917 | Interactor_type | Trans_regulator | |
| WBGene00003777 | Interactor_type | Trans_regulated | |||
| Expr_pattern | Expr3433 | ||||
| Interaction_summary | Marked difference was observed between wild-type embryos and PAR-2-depleted embryos in localization and dynamics of NMY-2::GFP and PAR-6::GFP between pseudocleavage and first cleavage. In wild-type embryos after pseudocleavage, NMY-2::GFP foci disappeared, and an anterior cap of fine NMY-2::GFP fibers formed in their place and persisted until metaphase. During this interval, anterior-directed flows of cortical myosin was observed terminating in a dense band at the posterior edge of the anterior NMY-2::GFP cap; analysis of fixed, stained embryos showed that this dense band of NMY-2 corresponded to a gap between anterior-localized PAR-3 and posterior-localized PAR-2. By contrast, in par-2 (RNAi) embryos, the disappearance of anterior NMY-2::GFP foci was followed by formation of a dense network of NMY-2::GFP fibers over most of the cortex. The appearance of posterior, ectopic NMY-2::GFP fibers was associated with a pronounced, posterior-directed cortical flow of NMY-2::GFP and a concurrent flow of PAR-6::GFP. This aberrant flow was sufficient to redistribute PAR-6::GFP to the posterior cortex by metaphase. These observations suggest that PAR-2 functions in wild-type embryos to inhibit NMY-2 from accumulating at the posterior cortex and thus prevents abnormal flows after pseudocleavage that would otherwise redistribute anterior PAR proteins to the posterior cortex. | ||||
| Detection_method | Reporter_gene | [nmy-2::gfp] translational fusion. | |||
| Regulation_result | Positive_regulate | Subcellular_localization | anterior | ||
| Interaction_RNAi | WBRNAi00063498 | ||||
| Paper | WBPaper00024378 | ||||
| Remark | par-3 mutants with uniformly distributed PAR-2 showed low, uniform levels of cortical NMY-2::GFP after pseudocleavage, while par-3;par-2(RNAi) embryos lacking PAR-2 had high, uniform levels of cortical NMY-2::GFP. RNAi: par-2 RNAi. |
