[C.elegansII] ct131 : germline P granules unstained by some(not all) monoclonal antibodies. 5% sterile, Mes if grown at 26C. Cloned: cosmid rescue (B0318). [SS]
P granules are not stained by K76 or OIC1D4, two anti-P-granule monoclonal antibodies. Mutants display sterility that is temperature-sensitive and has both a maternal and a nonmaternal component.
pgl-1 encodes a predicted RNA-binding protein that contains a number of C-terminal RGG box motifs; PGL-1 activity is required at high temperatures during midlarval to adult stages for postembryonic germline development and hence fertility, and also for proper formation of the germline-specific P granules; PGL-1 protein, supplied maternally and embryonically, is a constitutive P granule component and thus, its localization is cytoplasmic in oocytes and early embryos, while perinuclear in the germline blastomere P4 and proliferating germ cells of larvae and adults; pgl-1 mRNA is expressed exclusively in hermaphrodite and male germlines and is detectable at all stages of development, with highest levels seen in adults; PGL-1 localization to P granules requires wild-type activity of the predicted germline helicase GLH-1.
Enables RNA endonuclease activity; identical protein binding activity; and protein self-association. Involved in P granule organization and germ-line stem cell division. Located in P granule. Expressed in germ line.