WormBase Tree Display for Construct: WBCnstr00040176
expand all nodes | collapse all nodes | view schema
WBCnstr00040176 | Public_name | pBC1807 | |
---|---|---|---|
Summary | [Ptoe-2mKate2::tac-1] | ||
Driven_by_gene | WBGene00016971 | ||
Gene | WBGene00006381 | ||
Fusion_reporter | mkate2 | ||
Construction_summary | pBC1807 was generated by Gibson assembly (Gibson et al. 2009). Ptoe-2 was amplified using the primers NM1 (5'-gagctctggtaccctctagtcaaggTTATCTGTACCACAAATTCCTTG-3') and NM2 (5'-tgagctcggacatTTTTGACCTGAGGACATG-3'), and pBC1565 as a template. mKate2::tac-1 was obtained by PCR amplification using plasmid TMD34 (Pmex-5mKate2::tac-1::tbb-2 3'UTR; T. Mikeladze-Dvali, personal communication) as a template, and the primers NM3 (5'-cctcaggtcaaaaATGTCCGAGCTCATCAAG-3') and NM4 (5'-aattctacgaatgTTATGCATCCGTCGAAATAAC-3'). Similarly, pBC1565 was used as a template to amplify the unc-54 3'UTR using primers NM5 (5'-gacggatgcataaCATTCGTAGAATTCCAACTG-3') and NM6 (5'-agtccgtaatacgactcacttaaggAAACAGTTATGTTTGGTATATTGG-3'). These fragments were introduced into StuI-digested pCFJ909 (Frkjr-Jensen et al. 2014) using Gibson assembly to pBC1807 (Ptoe-2mKate2::tac-1). | ||
Used_for | Transgene_construct | WBTransgene00025429 | |
Reference | WBPaper00055255 |