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WormBase Tree Display for Construct: WBCnstr00040176

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Name Class

WBCnstr00040176Public_namepBC1807
Summary[Ptoe-2mKate2::tac-1]
Driven_by_geneWBGene00016971
GeneWBGene00006381
Fusion_reportermkate2
Construction_summarypBC1807 was generated by Gibson assembly (Gibson et al. 2009). Ptoe-2 was amplified using the primers NM1 (5'-gagctctggtaccctctagtcaaggTTATCTGTACCACAAATTCCTTG-3') and NM2 (5'-tgagctcggacatTTTTGACCTGAGGACATG-3'), and pBC1565 as a template. mKate2::tac-1 was obtained by PCR amplification using plasmid TMD34 (Pmex-5mKate2::tac-1::tbb-2 3'UTR; T. Mikeladze-Dvali, personal communication) as a template, and the primers NM3 (5'-cctcaggtcaaaaATGTCCGAGCTCATCAAG-3') and NM4 (5'-aattctacgaatgTTATGCATCCGTCGAAATAAC-3'). Similarly, pBC1565 was used as a template to amplify the unc-54 3'UTR using primers NM5 (5'-gacggatgcataaCATTCGTAGAATTCCAACTG-3') and NM6 (5'-agtccgtaatacgactcacttaaggAAACAGTTATGTTTGGTATATTGG-3'). These fragments were introduced into StuI-digested pCFJ909 (Frkjr-Jensen et al. 2014) using Gibson assembly to pBC1807 (Ptoe-2mKate2::tac-1).
Used_forTransgene_constructWBTransgene00025429
ReferenceWBPaper00055255