WormBase Tree Display for Construct: WBCnstr00018571
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| WBCnstr00018571 | Public_name | pCF1044 | |
|---|---|---|---|
| Summary | [Peat-4::rde-1::SL2::sid-1, Cbr-unc-119(+)] | ||
| Driven_by_gene | WBGene00001135 | ||
| Gene | WBGene00004323 | ||
| WBGene00004795 | |||
| Construction_summary | Clone = pCF1044. The bi-cistronic rde-1:SL2:sid-1 entry clone was generated using In-Fusion PCR cloning kit (Clonetech) in two steps: first, the 245 bp SL2-specific trans-splice site from the gpd-2/gpd-3 intergenic region was inserted upstream of the start codon of the sid-1 entry vector, then the rde-1 gene was inserted upstream of the SL2 site. The Peat-4 promoter entry clone was made by PCR amplification of 2582 bp upstream of the corresponding gene start site and cloned into pDONR-P4-P1R. The expression clone was generated using Gateway LR clonase II Plus (Life Technologies) and inserted into pCFJ150, a Gateway three-fragment compatible destination vector for MosSCI containing a C. briggsae unc-119 rescue fragment and genomic regions flanking the ttTi5605 Mos1 insertion. | ||
| Used_for | Transgene_construct | WBTransgene00019226 | |
| Reference | WBPaper00044483 |
