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WormBase Tree Display for Construct: WBCnstr00010870

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Name Class

WBCnstr00010870Other_nameExpr2614_Ex
Summary[pie-1::ceh-37-gfp]
Driven_by_geneWBGene00004027
GeneWBGene00000458
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[pie-1::ceh-37-gfp] translational fusion. The full-length ceh-37 ORF was substituted with the histone 2B ORF in the plasmid pJH4.52. pJH4.52, a plasmid that contains the pie-1 regulatory region, which drives GFP expression in early embryos. The construct mixed with PstI-digested N2 genomic DNA was then microinjected into mut-7 (pk204) mutants to avoid the germline suppression of transformed DNA and to prolong GFP expression over several generations.
Used_forTransgene_constructWBTransgene00028098
ReferenceWBPaper00006000