WormBase Tree Display for Construct: WBCnstr00010870
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WBCnstr00010870 | Other_name | Expr2614_Ex | |
---|---|---|---|
Summary | [pie-1::ceh-37-gfp] | ||
Driven_by_gene | WBGene00004027 | ||
Gene | WBGene00000458 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [pie-1::ceh-37-gfp] translational fusion. The full-length ceh-37 ORF was substituted with the histone 2B ORF in the plasmid pJH4.52. pJH4.52, a plasmid that contains the pie-1 regulatory region, which drives GFP expression in early embryos. The construct mixed with PstI-digested N2 genomic DNA was then microinjected into mut-7 (pk204) mutants to avoid the germline suppression of transformed DNA and to prolong GFP expression over several generations. | ||
Used_for | Transgene_construct | WBTransgene00028098 | |
Reference | WBPaper00006000 |