WormBase Tree Display for Construct: WBCnstr00007230
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WBCnstr00007230 | Summary | [Pcnc-2::mCherry; Pnlp-30::GFP; Pnlp-30::GFP; Pcol12:: DsRed] | |
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Driven_by_gene | WBGene00000556 | ||
WBGene00000601 | |||
WBGene00003768 | |||
Fusion_reporter | DsRed | ||
GFP | |||
mCherry | |||
Construction_summary | This construct contains 1.5 kilobases of genomic sequence upstream of the start codon of R09B5.3 and 252 base pairs downstream. It was obtained by PCR fusion using the vector pPD95.75::mCherry as the mCherry template. Transgenic worms were generated by microinjecting roughly 10 ng/l of the fusion PCR product, pnlp-30::GFP (20 ng/l) and the pcol12:: DsRed (70 ng/l) coinjection marker in N2 worms (Zugasti and Ewbank, 2009). | ||
Used_for | Transgene_construct | WBTransgene00007417 | |
Reference | WBPaper00032891 |