WormBase Tree Display for Antibody: WBAntibody00000277
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WBAntibody00000277 | Summary | Polyclonal rabbit antibodies against recombinant APR-1 protein. Antisera were affinity purified against the recombinant APR-1 fragment immobilized on PVDF membranes (Immobilon). | ||
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Public_name | [cgc3991]:apr-1_b | |||
Gene | WBGene00000156 | |||
Isolation | Original_publication | WBPaper00003991 | ||
Location | AH | |||
Clonality | Polyclonal | |||
Antigen | Protein | A 1.3-kb apr-1 cDNA fragment was isolated from a partial cDNA clone by PCR amplification with the primers 5 -TTTGGATCCACGGAGGATCAAGCGCAAATG-3 and 5 -TTTGGATCCGAACAGGCAGTCTTGACTTTCC-3 and cloned into the BamHI site of the Escherichia coli expression vector pDS6 to produce an APR-1 fragment consisting of amino acids 551 to 983 with an amino-terminal His6 tag. The His6::APR-1 fusion protein was produced in the E. coli strain M15, purified on NTA agarose according to the manufacturer's instructions (Qiagen), and used to immunize rats and rabbits. | ||
Animal | Rabbit | |||
Expr_pattern | Expr1046 | |||
Reference | WBPaper00003991 |