WormBase Tree Display for Rearrangement: eT1
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| eT1 | Name | Other_name | unc-72 | |
|---|---|---|---|---|
| eT1(III;V) | ||||
| eT1[unc-36] | ||||
| Variation | WBVar00143556 | |||
| Type | Translocation | |||
| Balances | III | To_right_end | ||
| Gene | WBGene00006772 | |||
| V | From_left_end | |||
| Gene | WBGene00006760 | |||
| Reference_strain | WBStrain00004210 | |||
| WBStrain00000586 | ||||
| Remark | cross-over sup left of V, right of III;'allele' of unc-36(III); e873 | |||
| [Edgley M] Reciprocal translocation. Suppresses recombination on left arm of LG V and right arm of LG III. Breaks in unc-36. Homozygous viable with Unc-36 phenotype. | ||||
| 20-bp sequences of either side of the eT1 breakpoints are as follows, eT1(V): aataatttttgaaacaaaaa-GTTTATCATCTCAAGACGTG; eT1(III): GAGCCAATCACTTACTGGTTAGTAATGATTCCAAATATGAGCCAATCACTTACTGGTTAGTAATGATTCCAAA-cttgattccaaatatgacat | Paper_evidence | WBPaper00027674 | ||
| Phenotype: Unc | ||||
| Summary: Reciprocal translocation, well characterized, extremely stable. Very effective balancer for left portion of LG V from left end through unc-23, and right portion of LG III from right end to unc-36. eT1(III) is LG V (left) translocated to LG III (left), disjoins from normal LG III. eT1(V) is LG III (right) translocated to LG V (right), disjoins from normal LG V. Zhao et al. reports the sequence of the physical breakpoint of eT1 on chromosome V (the breakpoint of eT1 chromosome III is in the unc-36 gene). | Paper_evidence | WBPaper00029020 | ||
| Growth characteristics: Original isolate homozygous viable with Unc-36 phenotype caused by the translocation breakpoint. Brood size in heterozygotes ~ 100, in homozygotes ~160. eT1 homozygotes can overgrow a population, especially in balanced strains carrying certain lethal mutations or deficiencies. | Paper_evidence | WBPaper00029020 | ||
| Handling: Easy to manipulate. Heterozygous male stock mate well. | Paper_evidence | WBPaper00029020 | ||
| Recommended use: General balancing, strain construction, strain maintenance, and mutant screens. | Paper_evidence | WBPaper00029020 | ||
| Isolation | Mutagen | 32P mutagenesis of N2 | ||
| Derived_rearrangement | eT1[bli-5(s277)] | |||
| eT1[dpy-11(s287)] | ||||
| eT1[sma-2(s262)] | ||||
| eT1[sma-3(e491)] | ||||
| eT1[unc-42(e270)] | ||||
| eT1[let-500(s2165)] | ||||
| eT1[him-5(e1467)] | ||||
| eT1[let-?(n886)] | ||||
| eT1[let-?(s1799)] | ||||
| Map | III | |||
| V | ||||
| Positive | Gene_inside | WBGene00006772 | ||
| Clone_inside | C34B10 | |||
| Mapping_data | 2_point | 309 | ||
| Strain (234) | ||||
| Reference (64) |
