WormBase Tree Display for Variation: WBVar00275490

WBVar00275490 Name: Public_name: zu127
  Sequence_details: SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Laboratory: JJ
    Status: Live
  Affects: Gene: WBGene00004027
    Interactor: WBInteraction000501692
      WBInteraction000519255
      WBInteraction000524133
  Genetics: Mapping_data: In_multi_point: 2115
        2116
        2118
        2119
        2120
        2121
        2122
        2123
  Description: Phenotype: WBPhenotype:0000050 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000414 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: P3-blastomere adopts an E-like fate (assayed via lineage analysis and polarization optics). C-blastomere adopts an MS-like fate. Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0002474 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
            WBbt:0003810 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
          Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: P3 blastomeres are isolated via laser ablation and scored for intestinal birefringence. C-blastomeres are isolated via laser ablation, followed by staining with 3NB12. Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
          Temperature: 22C Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000832 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: C-lineage in pie-1 mutants is abnormal. Additional blastomeres in 28-cell stage pie-1 embryos express the CeMyoD beta-galactosidase reporter Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0003810 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
          Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Embryos were also stained with LacZ Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
          Temperature: 22C Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001302 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: The restricted localization of P granules to germ cell precursors is altered. In pie-1 mutants P granules are distributed equally between both daughters of P3 (instead of just P4), and they continue to be distributed equally at each subsequent division Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: P-granule specific antibody staining Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001635 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: Mutants have large clusters of excess pharyngeal cells. These clusters are surrounded by a basement membrane and some of them contain grinder-like structures Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Penetrance: Incomplete: 78 percent Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: antibody staining with 3NB12 or 9.2.1 (stain pharyngeal-specific muscle cells) Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
          Temperature: 22C Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001636 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: pie-1 mutants appear to produce too many intestinal cells in intact as well as laser operated embryos Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Penetrance: Complete: 100 percent Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: P3 blastomeres were isolated via laser ablation and scored for intestinal birefringence Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
          Temperature: 16C, 25C Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0000472 Paper_evidence: WBPaper00002871
        Curator_confirmed: WBPerson2987
      WBPhenotype:0001642 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: Mutant embryos appear identical to wild-type embryos in terms of size, cleavage rate, and division axes up to the 28-cell stage (data not shown) Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
  Reference: WBPaper00002871
    WBPaper00001584
    WBPaper00022606
    WBPaper00021768
  Method: Allele